A new DNA combing method for biochemical analysis.
A new DNA combing method for biochemical analysis.
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DOI：
10.1016/j.ab.2010.01.021
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发表时间：
2010-05
影响因子：
2.9
通讯作者：
M. Oshige;K. Yamaguchi;S. Matsuura;H. Kurita;A. Mizuno;S. Katsura
中科院分区：
文献类型：
--
作者：
M. Oshige;K. Yamaguchi;S. Matsuura;H. Kurita;A. Mizuno;S. Katsura
本文介绍了一种用于生化反应分析的简单分子梳理方法--移动液滴法。在这种方法中，含有DNA分子的小液滴沿着倾斜的玻璃基板向下流动，这在空气、液滴和基板之间产生了一个移动的界面，从而拉伸DNA分子。该方法所需的样品溶液体积比其他已建立的梳理方法小得多，因此可以在各个领域中得到更广泛的应用。使用这种方法，λDNA分子被拉伸并吸附到玻璃基底上，并进行DNA聚合酶合成的单分子分析。
A simple molecular combing method for analysis of biochemical reactions, called the moving droplet method, has been developed. In this method, small droplets containing DNA molecules run down a sloped glass substrate, and this creates a moving interface among the air, droplet, and substrate that stretches the DNA molecules. This method requires a much smaller volume of sample solution than other established combing methods, allowing wider application in various fields. Using this method, λDNA molecules were stretched and absorbed to a glass substrate, and single-molecule analysis of DNA synthesis by DNA polymerases was performed.