Ultrastructure of the Membrana Limitans Interna after Dye-Assisted Membrane Peeling
Ultrastructure of the Membrana Limitans Interna after Dye-Assisted Membrane Peeling
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DOI：
10.1159/000331218
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发表时间：
2011-01-01
期刊：
影响因子：
2.6
通讯作者：
Dawczynski, Jens
中科院分区：
文献类型：
--
作者：
Brockmann, Tobias;Steger, Claudia;Dawczynski, Jens
本研究的目的是观察视网膜内界膜(内界膜，ILM)的超微结构，评价活性染料剥离膜后视网膜细胞层的变化。对25例(25只眼)黄斑裂孔手术患者，对12只吲哚青绿(ICG)和13只亮蓝(BBG)染色的ILM进行了光镜、透射电子显微镜和扫描电子显微镜观察。免疫组织化学方法检测两组ILM上的视网膜细胞碎片。比较ICG和BBG染色的细胞膜，BBG组中更大的细胞碎片出现的频率更高。因此，研究结果表明，ICG可以在较少的机械破坏下，加强ILM与底层视网膜的分离。一种可能的解释可以从已知的ICG的光敏性中看出，它导致ILM僵硬和收缩，但也产生视网膜有毒代谢产物。版权所有(C)2011 S.Karger AG，巴塞尔
The purpose of this study was to investigate the ultrastructure of the membrana limitans interna (internal limiting membrane, ILM) and to evaluate alterations to the retinal cell layers after membrane peeling with vital dyes. Twenty-five patients (25 eyes) who underwent macular hole surgery were included, whereby 12 indocyanine green (ICG)- and 13 brilliant blue G (BBG)-stained ILM were analyzed using light, transmission electron and scanning electron microscopy. Retinal cell fragments on the ILM were identified in both groups using immunohistochemistry. Comparing ICG- and BBG-stained membranes, larger cellular fragments were observed at a higher frequency in the BBG group. Thereby, the findings indicate that ICG permits an enhanced separation of the ILM from the underlying retina with less mechanical destruction. A possible explanation might be seen in the known photosensitivity of ICG, which induces a stiffening and shrinkage of the ILM but also generates retinal toxic metabolites. Copyright (C) 2011 S. Karger AG, Basel