Extracellular vesicle associated long non-coding RNAs functionally enhance cell viability.
Extracellular vesicle associated long non-coding RNAs functionally enhance cell viability.
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DOI：
10.1016/j.ncrna.2016.06.001
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发表时间：
2016-10
影响因子：
5
通讯作者：
Morris KV
中科院分区：
文献类型：
--
作者：
Hewson C;Capraro D;Burdach J;Whitaker N;Morris KV
细胞相互通信以创建微环境并共享资源。细胞通讯的一种途径是通过外泌体的作用。外泌体是一种细胞外囊泡，由一个细胞释放并被邻近细胞吸收。但外泌体如何促进细胞之间的通讯仍然很大程度上未知。我们在这里提供的证据表明，特定的长非编码 RNA 分子优先包装到外泌体中。我们还发现，这些外泌体相关非编码 RNA 中的一类特定类别通过与 L-乳酸脱氢酶 B (LDHB)、高迁移率族蛋白 17 (HMG-17) 和 CSF2RB（分别参与代谢、核小体结构和细胞信号传导的蛋白质）直接相互作用来功能性调节细胞活力。了解这种内源性细胞间途径、与外泌体相关非编码转录本及其相互作用域相互作用的蛋白质，不仅可以更好地理解细胞间相互作用，还可以更好地理解患者特异性细胞疗法中外泌体靶向方法的发展。
Cells communicate with one another to create microenvironments and share resources. One avenue by which cells communicate is through the action of exosomes. Exosomes are extracellular vesicles that are released by one cell and taken up by neighbouring cells. But how exosomes instigate communication between cells has remained largely unknown. We present evidence here that particular long non-coding RNA molecules are preferentially packaged into exosomes. We also find that a specific class of these exosome associated non-coding RNAs functionally modulate cell viability by direct interactions with l-lactate dehydrogenase B (LDHB), high-mobility group protein 17 (HMG-17), and CSF2RB, proteins involved in metabolism, nucleosomal architecture and cell signalling respectively. Knowledge of this endogenous cell to cell pathway, those proteins interacting with exosome associated non-coding transcripts and their interacting domains, could lead to a better understanding of not only cell to cell interactions but also the development of exosome targeted approaches in patient specific cell-based therapies.