Maturation of Mitochondrially Targeted Prx V Involves a Second Cleavage by Mitochondrial Intermediate Peptidase That Is Sensitive to Inhibition by H(2)O(2).
Maturation of Mitochondrially Targeted Prx V Involves a Second Cleavage by Mitochondrial Intermediate Peptidase That Is Sensitive to Inhibition by H(2)O(2).
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DOI：
10.3390/antiox10030346
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发表时间：
2021-02-25
期刊：
影响因子：
--
通讯作者：
Rhee SG
中科院分区：
文献类型：
--
作者：
Sim J;Park J;Woo HA;Rhee SG
Prx V mRNA contains two in-frame AUG codons, producing a long (L-Prx V) and short form of Prx V (S-Prx V), and mouse L-Prx V is expressed as a precursor protein containing a 49-amino acid N-terminal mitochondria targeting sequence. Here, we show that the N-terminal 41-residue sequence of L-Prx V is cleaved by mitochondrial processing peptidase (MPP) in the mitochondrial matrix to produce an intermediate Prx V (I-Prx V) with a destabilizing phenylalanine at its N-terminus, and further, that the next 8-residue sequence is cleaved by mitochondrial intermediate peptidase (MIP) to convert I-Prx V to a stabilized mature form that is identical to S-Prx V. Further, we show that when mitochondrial H2O2 levels are increased in HeLa cells using rotenone, in several mouse tissues by deleting Prx III, and in the adrenal gland by deleting Srx or by exposing mice to immobilized stress, I-Prx V accumulates transiently and mature S-Prx V levels decrease in mitochondria over time. These findings support the view that MIP is inhibited by H2O2, resulting in the accumulation and subsequent degradation of I-Prx V, identifying a role for redox mediated regulation of Prx V proteolytic maturation and expression in mitochondria.
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影响因子：
4.8
作者：
Knoops, B;Clippe, A;Bernard, A
通讯作者：
Bernard, A
DOI：
10.1016/j.bbaexp.2007.05.004
发表时间：
2007-07-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
影响因子：
--
作者：
Nguyen-nhu, Nhu Tien;Berck, Jehanne;Knoops, Bernard
通讯作者：
Knoops, Bernard
影响因子：
7
作者：
Park, Jiyoung;Lee, Eun Gyeong;Woo, Hyun Ae
通讯作者：
Woo, Hyun Ae
影响因子：
4.8
作者：
Gomes, Fernando;Palma, Flavio Romero;Netto, Luis E. S.
通讯作者：
Netto, Luis E. S.
影响因子：
12.3
作者：
Eldomery MK;Akdemir ZC;Vögtle FN;Charng WL;Mulica P;Rosenfeld JA;Gambin T;Gu S;Burrage LC;Al Shamsi A;Penney S;Jhangiani SN;Zimmerman HH;Muzny DM;Wang X;Tang J;Medikonda R;Ramachandran PV;Wong LJ;Boerwinkle E;Gibbs RA;Eng CM;Lalani SR;Hertecant J;Rodenburg RJ;Abdul-Rahman OA;Yang Y;Xia F;Wang MC;Lupski JR;Meisinger C;Sutton VR
通讯作者：
Sutton VR