A system biology approach for understanding the miRNA regulatory network in colon rectal cancer
A system biology approach for understanding the miRNA regulatory network in colon rectal cancer
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DOI：
10.1504/ijdmb.2015.066332
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发表时间：
2015-01-01
影响因子：
0.3
通讯作者：
Palakal, Mathew
中科院分区：
文献类型：
--
作者：
Pradhan, Meeta;Nagulapalli, Kshithija;Palakal, Mathew
在本文中，我们提出了一个系统生物学的方法来理解的miRNA调控网络在结肠直肠癌。通过对相关文献的挖掘，初步获得了一组与结肠直肠癌（CRC）相关的基因。从三个数据库获得癌症相关miRNA的初始集合：miRBase、miRWalk、Targetscan和GEO微阵列实验。然后使用第一原理方法来生成全局miRNA-基因网络。使用拓扑和子图分析确定了全球miRNA-基因网络中的重要miRNA和相关转录因子。鉴定了11种新的miRNAs，并进一步分析了其中3种新的miRNAs，hsa-miR-630、hsa-miR-100和hsa-miR-99 a，以阐明它们在CRC中的作用。所提出的方法有效地利用了文献数据，并能够显示新的，显着的miRNA转录在CRC中的关联。
In this paper we present a systems biology approach to the understanding of the miRNA-regulatory network in colon rectal cancer. An initial set of significant genes in Colon Rectal Cancer (CRC) were obtained by mining relevant literature. An initial set of cancer-related miRNAs were obtained from three databases: miRBase, miRWalk, Targetscan and GEO microarray experiment. First principle methods were then used to generate the global miRNA-gene network. Significant miRNAs and associated transcription factors in the global miRNA-gene network were identified using topological and sub-graph analyses. Eleven novel miRNAs were identified and three of the novel miRNAs, hsa-miR-630, hsa-miR-100 and hsa-miR-99a, were further analysed to elucidate their role in CRC. The proposed methodology effectively made use of literature data and was able to show novel, significant miRNA-transcription associations in CRC.