Ability of Lactobacillus and Propionibacterium strains to remove aflatoxin B1 from the chicken duodenum
Ability of Lactobacillus and Propionibacterium strains to remove aflatoxin B1 from the chicken duodenum
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DOI：
10.4315/0362-028x-63.4.549
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发表时间：
2000-04-01
影响因子：
2
通讯作者：
Ahokas, J
中科院分区：
文献类型：
--
作者：
El-Nezami, H;Mykkänen, H;Ahokas, J
用鸡肠道环流技术在体内测试了鼠李糖乳杆菌菌株GG和LC-705从肠腔液体培养上清液中去除黄曲霉毒素B(1)的能力。在这项研究中，鼠李糖GG菌株在1分钟内将腔液中可溶部分中的AFB(1)浓度降低了54%。在相似条件下，该菌株与黄曲霉毒素B(1)的结合效率高于鼠李糖乳杆菌菌株LC-705(P<；0.05)，后者对黄曲霉毒素B(1)的降解率为44%。此外，还测定了AFB(1)在肠道组织中的蓄积。在鼠李糖菌株GG存在的情况下，肠道组织对AFB(1)的摄取减少了74%，而在弗罗德赖氏丙酸杆菌SSP的情况下，分别减少了63%和37%。鼠李糖菌株GG和L与鼠李糖菌株LC-705和黄曲霉毒素B(1)在体外形成的复合体在管腔条件下稳定1h。
The ability of Lactobacillus rhamnosus strains GG and LC-705 to remove AFB(1) from the intestinal luminal liquid medium has been tested in vivo using a chicken intestinal loop technique. In this study, the GG strain of L. rhamnosus decreased AFB(1) concentration by 54% in the soluble fraction of the luminal fluid within 1 min. This strain was more efficient in binding AFB(1) compared with L. rhamnosus strain LC-705 (P < 0.05) that removed 44% of AFB(1) under similar conditions. Accumulation of AFB(1) into the intestinal tissue was also determined. There was a 74% reduction in the uptake of AFB(1) by the intestinal tissue, in the presence of L. rhamnosus strain GG compared with 63% and 37% in the case of propionibacterium freudenreichii ssp. shermanii JS and L. rhamnosus strain LC-705, respectively, The complexes formed in vitro between either L. rhamnosus strain GG or L, rhamnosus strain LC-705 and AFB(1) were stable under the luminal conditions for a period of 1 h.