Model membrane platforms to study protein-membrane interactions
Model membrane platforms to study protein-membrane interactions
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DOI：
10.3109/09687688.2012.700490
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发表时间：
2012-08-01
影响因子：
--
通讯作者：
Schwille, Petra
中科院分区：
文献类型：
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作者：
Sezgin, Erdinc;Schwille, Petra
蛋白质与脂质膜之间的功能性相互作用是细胞膜的基本特征之一。在活细胞中定量研究这些相互作用的主要挑战是，如果不是不可能的话，也很难同时控制大量的相关成分。因此，非常需要简化但仍然足够详细的模型系统来研究生物过程的关键组成部分。为了特别关注膜蛋白和脂质之间的相互作用，已经引入了几种膜模型，这些模型在不同程度上概括了生物膜的复杂性和物理化学性质。本文综述了目前应用最广泛的最小模型膜系统，即支撑脂质双层（SLB）、巨单层囊泡（GUV）和巨质膜囊泡（GPMV）及其在蛋白质-膜相互作用研究中的应用。
Constituting functional interactions between proteins and lipid membranes is one of the essential features of cellular membranes. The major challenge of quantitatively studying these interactions in living cells is the multitude of involved components that are difficult, if not impossible, to simultaneously control. Therefore, there is great need for simplified but still sufficiently detailed model systems to investigate the key constituents of biological processes. To specifically focus on interactions between membrane proteins and lipids, several membrane models have been introduced which recapitulate to varying degrees the complexity and physicochemical nature of biological membranes. Here, we summarize the presently most widely used minimal model membrane systems, namely Supported Lipid Bilayers (SLBs), Giant Unilamellar Vesicles (GUVs) and Giant Plasma Membrane Vesicles (GPMVs) and their applications for protein-membrane interactions.