D1 receptor modulation of action potential firing in a subpopulation of layer 5 pyramidal neurons in the prefrontal cortex.
D1 receptor modulation of action potential firing in a subpopulation of layer 5 pyramidal neurons in the prefrontal cortex.
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DOI：
10.1523/jneurosci.1367-12.2012
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发表时间：
2012-08-01
期刊：
影响因子：
--
通讯作者：
Carter AG
中科院分区：
文献类型：
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作者：
Seong HJ;Carter AG
多巴胺在前额叶皮层的调节对认知加工很重要，并在多种神经精神疾病中被破坏。D1受体的激活被认为是通过增强锥体神经元的放电特性来实现工作记忆。然而，这些受体仅在前额皮质中少量表达，它们如何影响单个神经元仍然未知。本文研究了小鼠前额皮质急性切片第5层锥体神经元D1受体的调节。利用全细胞记录和双光子显微镜，我们发现表达D1受体的神经元具有独特的形态和生理特性。然后，我们证明了这些受体的激活选择性地通过PKA通路增强了这些神经元的放电。这一发现强大的D1受体调节仅在一个亚群神经元具有重要意义的认知功能和疾病。
Dopamine modulation in the prefrontal cortex is important for cognitive processing and disrupted in diverse neuropsychiatric diseases. Activation of D1 receptors is thought to enable working memory by enhancing the firing properties of pyramidal neurons. However, these receptors are only sparsely expressed in the prefrontal cortex, and how they impact individual neurons remains unknown. Here we study D1 receptor modulation of layer 5 pyramidal neurons in acute slices of the mouse prefrontal cortex. Using whole-cell recordings and two-photon microscopy, we show that neurons expressing D1 receptors have unique morphological and physiological properties. We then demonstrate that activation of these receptors selectively enhances the firing of these neurons by signaling via the PKA pathway. This finding of robust D1 receptor modulation in only a subpopulation of neurons has important implications for cognitive function and disease.