Detection of Microvasculature in Rat Hind Limb Using Synchrotron Radiation
Detection of Microvasculature in Rat Hind Limb Using Synchrotron Radiation
复制标题
使用同步辐射检测大鼠后肢微脉管系统
DOI：
10.1016/j.jss.2010.05.015
复制
发表时间：
2010-11-01
影响因子：
2.2
通讯作者：
Zhang, Xiangman
中科院分区：
文献类型：
--
作者：
Lu, Weifeng;Dong, Zhihui;Zhang, Xiangman
背景。新型x射线微血管成像技术和第三代同步辐射微计算机断层扫描技术为四肢微血管成像开辟了新的视角。本实验旨在利用微血管造影技术观察大鼠后肢深层微血管结构，并与常规方法进行比较。材料与方法。采用Sprague Dawley大鼠10只（5只/组）进行体内和离体实验。在体、离体进行血管造影，并与常规方法进行对比。采用同步辐射微计算机断层扫描（SR mu CT）显示大鼠后肢血管的三维形态。微血管造影技术可以清晰地描绘大鼠肢体血管，最小可见血管直径约为9 mm，血管造影评分高于常规x线。此外，通过SR mu ct显示的肢体血管三维渲染图，可以在微米尺度上对血管网络进行定义和分析。因此，同步辐射微血管造影和磁共振CT为观察肢体微血管提供了一种实用有效的手段，可用于评估下肢血管生成情况。(C) 2010爱思唯尔公司版权所有。
Background. New X-ray microangiography and third-generation synchrotron radiation-based micro-computed tomography have opened new perspectives for microvascular imaging of extremity. Here we aimed to visualize deep-level microvascular structure in rat hind limb by microangiographic technique, and compare images with those by conventional method.Materials and Methods. A total of 10 Sprague Dawley rats were used for in vivo and ex vivo study (five rats/group). Microangiography in vivo and ex vivo was performed and images were compared with those by conventional method. Synchrotron radiation-based micro-computed tomography (SR mu CT) was also performed to reveal three-dimensional (3D) morphology of the blood vessel in rat hind limb.Results. By microangiographic technique, blood vessels in the rat limb could be clearly depicted with the minimum visualized blood vessel about 9 mm in diameter, and higher angiographic scores were achieved than those by conventional X-ray. In addition, the vascular network could be defined and analyzed at the micrometer scale from the 3D renderings of limb vessel as shown by SR mu CT.Conclusions. Synchrotron radiation-based microangiography and SR mu CT thus provided a practical and effective means to observe the microvasculature of limbs, which might be useful in assessment of angiogenesis in lower limbs. (C) 2010 Elsevier Inc. All rights reserved.