Novel phosphorylated forms of E2F-1 transcription factor bind to the retinoblastoma protein in cells overexpressing an E2F-1 cDNA.
Novel phosphorylated forms of E2F-1 transcription factor bind to the retinoblastoma protein in cells overexpressing an E2F-1 cDNA.
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新型磷酸化形式的 E2F-1 转录因子与过表达 E2F-1 cDNA 的细胞中的视网膜母细胞瘤蛋白结合。
DOI：
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发表时间：
1997
期刊：
影响因子：
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通讯作者：
T. Sladek
中科院分区：
文献类型：
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作者：
X. Yang;T. Sladek
在过表达编码 E2F-1 转录因子的外源 cDNA 的细胞中，经过变性蛋白凝胶电泳和使用 E2F-1 特异性抗体进行免疫印迹后，检测到多达 8 个紧密迁移的蛋白条带。对照细胞未过度表达 E2F-1 cDNA，仅包含四个 E2F-1 特异性条带。用 lambda-磷酸酶对对照细胞和 E2F-1 过表达细胞的蛋白质提取物进行预处理，消除了除凝胶上迁移最快的单条带外的所有 E2F-1 特异性条带。这些数据表明，多个蛋白条带是E2F-1蛋白的差异磷酸化形式，并且表明在过表达E2F-1蛋白的细胞中存在新的、更高磷酸化的E2F-1形式。此外，对过表达 E2F-1 cDNA 的细胞中的视网膜母细胞瘤 (pRb) 蛋白进行免疫沉淀显示，新型高度磷酸化的 E2F-1 形式优先进行免疫共沉淀，表明 pRb 优先与这些 E2F-1 形式结合。
In cells overexpressing an exogenous cDNA encoding the E2F-1 transcription factor, as many as eight closely-migrating protein bands were detected after denaturing protein gel electrophoresis and immunoblotting with an E2F-1-specific antibody. Control cells, not overexpressing an E2F-1 cDNA, contained only four E2F-1 specific bands. Pretreatment of protein extracts, from both control and E2F-1 overexpressing cells, with lambda-phosphatase eliminated all E2F-1-specific bands except the single band migrating most rapidly on the gels. These data demonstrated that the multiple protein bands were differentially-phosphorylated forms of E2F-1 protein and showed that novel, more highly phosphorylated E2F-1 forms were present in cells overexpressing the E2F-1 protein. In addition, immunoprecipitation of the retinoblastoma (pRb) protein from cells overexpressing the E2F-1 cDNA showed that the novel, highly phosphorylated E2F-1 forms were preferentially coimmunoprecipitated, indicating that pRb bound preferentially to these E2F-1 forms.
影响因子：
4.4
作者：
SAITO, M;HELIN, K;LOOK, AT
通讯作者：
LOOK, AT