Metabolic engineering of the pentose phosphate pathway for enhanced limonene production in the cyanobacterium Synechocysti s sp. PCC 6803.
Metabolic engineering of the pentose phosphate pathway for enhanced limonene production in the cyanobacterium Synechocysti s sp. PCC 6803.
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DOI：
10.1038/s41598-017-17831-y
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发表时间：
2017-12-13
影响因子：
4.6
通讯作者：
Pakrasi HB
中科院分区：
文献类型：
--
作者：
Lin PC;Saha R;Zhang F;Pakrasi HB
类异戊二烯是多种天然化合物，有多种用途，如药物、香料和溶剂。植物中类异戊二烯的低产率使得它们难以具有成本效益的生产，并且化学合成复杂的类异戊二烯是不切实际的。微生物生产类异戊二烯被认为是一种很有前途的提高产量的方法。在这项研究中，我们设计了蓝藻模型聚囊藻sp. PCC 6803，用于可持续生产具有商业价值的类异戊二烯柠檬烯。从植物薄荷和柠檬柑橘中提取的柠檬烯合成酶在蓝藻中表达，用于生产柠檬烯。用柠檬烯合成酶生产柠檬烯比用柠檬草生产柠檬烯高2倍。为了提高类异戊二烯的产量，应用OptForce应变设计算法对Synechocystis 6803进行了计算应变设计。基于该算法建议的代谢干预，对戊糖磷酸途径中的基因（核糖5-磷酸异构酶和核酮糖5-磷酸3-外聚酶）进行过表达，并对植物冷杉（Abies grandis）中的香叶二磷酸合成酶进行表达，优化柠檬烯生物合成途径。优化后的菌株产柠檬烯6.7 mg/L，产量提高2.3倍。因此，本研究提出了一种可行的策略来设计蓝藻进行光合作用生产类异戊二烯。
Isoprenoids are diverse natural compounds, which have various applications as pharmaceuticals, fragrances, and solvents. The low yield of isoprenoids in plants makes them difficult for cost-effective production, and chemical synthesis of complex isoprenoids is impractical. Microbial production of isoprenoids has been considered as a promising approach to increase the yield. In this study, we engineered the model cyanobacterium Synechocystis sp. PCC 6803 for sustainable production of a commercially valuable isoprenoid, limonene. Limonene synthases from the plants Mentha spicata and Citrus limon were expressed in cyanobacteria for limonene production. Production of limonene was two-fold higher with limonene synthase from M. spicata than that from C. limon. To enhance isoprenoid production, computational strain design was conducted by applying the OptForce strain design algorithm on Synechocystis 6803. Based on the metabolic interventions suggested by this algorithm, genes (ribose 5-phosphate isomerase and ribulose 5-phosphate 3-epimerase) in the pentose phosphate pathway were overexpressed, and a geranyl diphosphate synthase from the plant Abies grandis was expressed to optimize the limonene biosynthetic pathway. The optimized strain produced 6.7 mg/L of limonene, a 2.3-fold improvement in productivity. Thus, this study presents a feasible strategy to engineer cyanobacteria for photosynthetic production of isoprenoids.
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影响因子：
48
作者：
Gibson, Daniel G.;Young, Lei;Smith, Hamilton O.
通讯作者：
Smith, Hamilton O.
影响因子：
4.8
作者：
Estévez, JM;Cantero, A;León, P
通讯作者：
León, P
影响因子：
4.8
作者：
Hosfield, DJ;Zhang, YM;Finn, J
通讯作者：
Finn, J
DOI：
10.1073/pnas.1200074109
发表时间：
2012-04-17
影响因子：
11.1
作者：
Lan, Ethan I.;Liao, James C.
通讯作者：
Liao, James C.
影响因子：
4.8
作者：
Bohlmann, J;Steele, CL;Croteau, R
通讯作者：
Croteau, R