Membrane ruffles in cell migration: indicators of inefficient lamellipodia adhesion and compartments of actin filament reorganization
Membrane ruffles in cell migration: indicators of inefficient lamellipodia adhesion and compartments of actin filament reorganization
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DOI：
10.1016/j.yexcr.2004.08.034
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发表时间：
2005-01-01
影响因子：
3.7
通讯作者：
Kirfel, G
中科院分区：
文献类型：
--
作者：
Borm, B;Requardt, RP;Kirfel, G
在上皮细胞迁移过程中，相差显微镜可观察到膜皱褶，其在板状伪足的前缘出现暗波，向主细胞体向心移动。尽管通常使用术语膜皱褶，但它们的结构、分子组成和导致其形成的机制在很大程度上仍然未知。在这里，我们表明，膜皱褶不同的底层细胞片层的更密集的肌动蛋白纤维束，富含肌动蛋白交联剂细丝蛋白和埃兹蛋白，指向一个特定的捆绑过程的基础上，这些交联剂。磷酸化，即失活，cofilin在膜皱褶的积累表明，它们是抑制肌动蛋白丝营业额的隔间。高Rac 1和低RhoA活性的条件下，发现次优的整合素-配体相互作用与低板状伪足持久性，低效的迁移，和高皱褶率。基于这些发现，我们定义膜皱褶作为不同的车厢的特定组成，形成作为一个结果，效率低下的lamellipodia粘附。(C)2004年爱思唯尔公司All rights reserved.
During epithelial cell migration, membrane ruffles can be Visualized by phase contrast microscopy as dark waves arising at the leading edge of lamellipodia that move centripetally toward the main cell body. Despite the common use of the term membrane ruffles, their structure, molecular composition, and the mechanisms leading to their formation remained largely unknown. We show here that membrane ruffles differ from the underlying cell lamella by more densely packed bundles of actin filaments that are enriched in the actin cross-linkers filamin and ezrin, pointing to a specific bundling process based on these cross-linkers. The accumulation of phosphorylated, that is, inactivated, cofilin in membrane ruffles suggests that they are compartments of inhibited actin filament turnover. High Rac1 and low RhoA activities were found under conditions of suboptimal integrin-ligand interaction correlating with low lamellipodia persistence, inefficient migration, and high ruffling rates. Based on these findings, we define membrane ruffles as distinct compartments of specific composition that form as a consequence of inefficient lamellipodia adhesion. (C) 2004 Elsevier Inc. All rights reserved.