Dermal matrix as a carrier for in vivo delivery of human adipose-derived stem cells
Dermal matrix as a carrier for in vivo delivery of human adipose-derived stem cells
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DOI：
10.1016/j.biomaterials.2007.11.026
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发表时间：
2008-04-01
期刊：
影响因子：
14
通讯作者：
Alt, Eckhard U.
中科院分区：
文献类型：
--
作者：
Altman, Andrew M.;Matthias, Nadine;Alt, Eckhard U.
本研究的目的是评估脱细胞真皮基质作为载体将干细胞输送到小鼠皮肤损伤模型软组织缺损部位的潜力，并确定通过这种方法输送的干细胞的潜力成功移植、存活和局部分化。我们发现，通过该基质递送的脂肪源性干细胞在体内植入后存活，沿着血管内皮、成纤维细胞和表皮上皮谱系自发分化，并显著改善伤口愈合。此外，移植细胞的器官调查显示，没有证据表明全身分布超出皮肤伤口部位，表明脂肪来源的干细胞-真皮基质构建体提供了一种新的和有效的方法，解剖学上集中的细胞治疗。总之，干细胞接种真皮基质是一种有效的手段，有针对性地在体内细胞输送增强软组织再生。(c)2007爱思唯尔有限公司保留所有权利。
The aim of the present study was to evaluate the potential of acellular dermal matrix as a carrier for delivery of stem cells to the site of soft tissue defect in a murine skin injury model and to determine the potential of stem cells delivered via such an approach to successfully engraft, survive and differentiate locally. We showed that adipose-derived stem cells delivered via this matrix survived after in vivo engraftment, spontaneously differentiated along vascular endothelial, fibroblastic and epidermal epithelial lineages and significantly improved wound healing. Furthermore, an organ survey for transplanted cells showed no evidence of a systemic distribution beyond the cutaneous wound site, indicating that the adipose-derived stem cell-dermal matrix construct provides a novel and effective method for anatomically focused cellular therapy. In conclusion, stem cell-seeded dermal matrix is an effective means for targeted in vivo cell delivery for enhanced soft tissue regeneration. (c) 2007 Elsevier Ltd. All rights reserved.