Astrocytes from mouse brain slices express ClC-2-mediated Cl- currents regulated during development and after injury
Astrocytes from mouse brain slices express ClC-2-mediated Cl- currents regulated during development and after injury
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DOI：
10.1016/s1044-7431(03)00080-0
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发表时间：
2003-08-01
影响因子：
3.5
通讯作者：
Kettenmann, H
中科院分区：
文献类型：
--
作者：
Makara, JK;Rappert, A;Kettenmann, H
氯离子通道对于星形胶质细胞的容量调节和K+缓冲是重要的。我们证明了超极化激活的Cl-电流在急性切片中的星形胶质细胞亚群中的功能表达，或在从GFAP/EGFP转基因动物的成年脑中选择性标记星形胶质细胞的新鲜分离后的功能表达。当Na ~+和K ~+被NMDG（+）和Cs ~+取代时，在细胞外和细胞内均能观察到负膜电位下的内向电流。该电流显示出与培养的星形胶质细胞中的Cl-电流特征相同的特征：在-40 mV以下的负电位下，其激活时间依赖性，在1 s内未显示失活，并且被亚微摩尔浓度的Cd 2+可逆地抑制。在来自ClC-2敲除小鼠的星形胶质细胞中未检测到电流，表明ClC-2氯离子通道产生电导。电流密度显着较低的相应人口的星形胶质细胞分离出未成熟的大脑和反应性星形胶质细胞内的病变部位。(C)2003 Elsevier Science（美国）。All rights reserved.
Chloride channels are important for astrocytic volume regulation and K+ buffering. We demonstrate functional expression of a hyperpolarization-activated Cl- current in a subpopulation of astrocytes in acute slices or after fresh isolation from adult brain of GFAP/EGFP transgenic animals in which astrocytes are selectively labeled. When Na+ and K+ were substituted with NMDG(+) and Cs+ in extra- and intracellular solutions, an inward current was observed at negative membrane potentials. The current displayed features as described for a Cl- current characterized in cultured astrocytes: it activated time dependently at potentials negative to -40 mV, displayed no inactivation within 1 s, and was inhibited reversibly by submicromolar concentrations of Cd2+. The current was not detectable in astrocytes from ClC-2 knockout mice, indicating that the ClC-2 chloride channel generated the conductance. Current density was significantly lower in a corresponding population of astrocytes isolated from immature brain and in reactive astrocytes within a lesion site. (C) 2003 Elsevier Science (USA). All rights reserved.