Impact of engineered surface microtopography on biofilm formation of Staphylococcus aureus
Impact of engineered surface microtopography on biofilm formation of Staphylococcus aureus
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DOI：
10.1116/1.2751405
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发表时间：
2007-06-01
期刊：
影响因子：
2.1
通讯作者：
Brennana, Anthony B.
中科院分区：
文献类型：
--
作者：
Chung, Kenneth K.;Schumacher, James F.;Brennana, Anthony B.
留置医疗设备的表面可能会被人类病原体定植，这些病原体可能会形成生物膜并导致感染。在大多数情况下，这些生物膜对抗菌治疗具有抵抗力，最终需要移除或更换该装置。在聚二甲基硅氧烷弹性体(PDMSe)上设计了一种基于鲨鱼皮肤的工程表面微地形，Sharklet AF(TM)可以在不使用杀菌剂的情况下破坏细菌生物膜的形成。在21天的过程中，Sharklet AF(TM)PDMSe与平滑PDMSe进行了金黄色葡萄球菌生物被膜形成的测试。光滑表面在第7天出现早期生物膜菌落，在第14天出现成熟生物膜，而地形表面直到第21天才显示出早期生物膜定植的证据。第14天，金黄色葡萄球菌在光滑表面的覆盖率平均值为54%，而Sharklet AF表面的覆盖率为7%(p<；0.01)。这些结果表明，用Sharklet AF T工程地形对留置医疗器械和暴露的无菌表面进行表面改性可能是破坏金黄色葡萄球菌生物被膜形成的一种有效解决方案。(C)2007年美国真空协会。
The surface of an indwelling medical device can be colonized by human pathogens that can form biofilms and cause infections. In most cases, these biofilms are resistant to antimicrobial therapy and eventually necessitate removal or replacement of the device. An engineered surface microtopography based on the skin of sharks, Sharklet AF(TM), has been designed on a poly (dimethyl siloxane) elastomer (PDMSe) to disrupt the formation of bacterial biofilms without the use of bactericidal agents. The Sharklet AF(TM) PDMSe was tested against smooth PDMSe for biofilm formation of Staphylococcus aureus over the course of 21 days. The smooth surface exhibited early-stage biofilm colonies at 7 days and mature biofilms at 14 days, while the topographical surface did not show evidence of early biofilm colonization until day 21. At 14 days, the mean value of percent area coverage of S. aureus on the smooth surface was 54% compared to 7% for the Sharklet AF T surface (p < 0.01). These results suggest that surface modification of indwelling medical devices and exposed sterile surfaces with the Sharklet AF T engineered topography may be an effective solution in disrupting biofilm formation of S. aureus. (C) 2007 American Vacuum Society.