Mechanical induction of twist in the Drosophila foregut/stomodeal primordium
Mechanical induction of twist in the Drosophila foregut/stomodeal primordium
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DOI：
10.1016/s0960-9822(03)00576-1
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发表时间：
2003-08-19
期刊：
影响因子：
9.2
通讯作者：
Farge, E
中科院分区：
文献类型：
--
作者：
Farge, E
背景：形态发生运动受发育基因表达的密切调控。在这里，我研究是否发育基因的表达可以反过来被机械调节形态发生运动。我分析了机械应力对Twist表达的影响，Twist通常只在细胞胚盘胚胎的最腹侧细胞中表达，受背侧形态梯度的控制。在胚胎发生原肠胚形成（第7阶段），扭曲也表示在前前肠和stomodeal primordia.Results：提交的早期果蝇胚胎短暂的10%单轴侧向变形诱导扭曲的异位表达在整个背腹轴和结果在腹侧的胚胎。这种诱导是独立的背梯度，并触发机械诱导的犰狳核易位。我还表明，扭曲不表达在前前肠和stomodeal，原基在第7阶段的突变体，阻止胚带延伸的形态发生运动。因为我可以通过温和地压缩这些细胞来拯救突变体，所以我的解释是，通常由生殖带延伸引起的气孔器细胞压缩诱导了Twist的表达。相应地，野生型胚胎背细胞的激光消融放松了气孔器细胞的压缩，并减少了气孔器原基中Twist的表达。我还表明，在这些细胞中的扭曲的诱导依赖于Armadillo.Conclusions的核转位：我建议，前肠的形成是机械诱导的生殖带延伸的运动，通过诱导扭曲的表达在stomodeal细胞。
Background: Morphogenetic movements are closely regulated by the expression of developmental genes. Here I examine whether developmental gene expression can in turn be mechanically regulated by morphogenetic movements. I have analyzed the effects of mechanical stress on the expression of Twist, which is normally expressed only in the most ventral cells of the cellular blastoderm embryo under the control of the Dorsal morphogen gradient. At embryogenesis gastrulation (stage 7), Twist is also expressed in the anterior foregut and stomodeal primordia.Results: Submitting the early Drosophila embryo to a transient 10% uniaxial lateral deformation induces the ectopic expression of Twist around the entire dorsal-ventral axis and results in the ventralization of the embryo. This induction is independent of the Dorsal gradient and is triggered by mechanically induced Armadillo nuclear translocation. I also show that Twist is not expressed in the anterior foregut and stomodeal,primordia at stage 7 in mutants that block the morphogenetic movement of germ-band extension. Because I can rescue the mutants with gentle compression of these cells, my interpretation is that the stomodeal-cell compression normally caused by the germ-band extension induces the expression of Twist. Correspondingly, laser ablation of dorsal cells in wild-type embryos relaxes stomodeal cell compression and reduces Twist expression in the stomodeal primordium. I also demonstrate that the induction of Twist in these cells depends on the nuclear translocation of Armadillo.Conclusions: I propose that anterior-gut formation is mechanically induced by the movement of germ-band extension through the induction of Twist expression in stomodeal cells.