Dectin-2 polymorphism associated with pulmonary cryptococcosis in HIV-uninfected Chinese patients
Dectin-2 polymorphism associated with pulmonary cryptococcosis in HIV-uninfected Chinese patients
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DOI：
10.1093/mmy/myv043
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发表时间：
2015-11-01
期刊：
影响因子：
2.9
通讯作者：
Zhu, Li-Ping
中科院分区：
文献类型：
--
作者：
Hu, Xiu-Ping;Wang, Rui-Ying;Zhu, Li-Ping
Dectin-2是一种C型凝集素受体，可以识别真菌的关键结构，参与肺部真菌感染后的宿主免疫反应。我们的目的是在一系列未感染人类免疫缺陷病毒(HIV)的中国患者中调查Dectin-2基因多态性与隐球菌病的关系。在这项病例对照研究中，共包括251名隐球菌病患者和464名健康对照。本研究选择了一个位于Dectin-2基因5‘侧翼区的标签-单核苷酸多态(SNP)(Rs11045418)，并对其进行了基因分型。251例患者中，脑膜炎108例(43%)，其中健康73例(67.7%)，肺部感染74例(29.5%)，健康49例(66.2%)，神经和肺部感染69例(27.5%)，免疫功能正常38例(55.1%)。肺隐球菌病患者143例(74+69)，隐球菌性脑膜炎患者177例(108+69)，分别与对照组比较。143例肺部感染患者的3份样本未能进行基因分型。在优势模式下，86例免疫功能正常的肺部感染患者与对照组相比差异有统计学意义(C/T vs.T/T+C/C；OR，0.59；95%CI，0.37~0.94；P，0.026)。在强显性模式下，总体肺部感染患者与对照组之间有相似但无显著差异(OR，0.77；95%CI，0.52-1.12；P，0.17)。在对照组和隐球菌性脑膜炎患者之间没有发现这样的差异。我们的研究首次表明Dectin-2与肺隐球菌病之间存在遗传关联。
Dectin-2 is a C-type lectin receptor that can recognize critical structures of fungi and involve in the host immune response after pulmonary fungal infections. We aimed to investigate the association between Dectin-2 genetic polymorphisms and cryptococcosis among a series of human immunodeficiency virus (HIV)-uninfected Chinese patients. In this case control study, a total of 251 patients with cryptococcosis and 464 healthy controls were included. One tag-single nucleotide polymorphism (SNP) (rs11045418) located at 5'-flanking region of Dectin-2 gene was selected and genotyped in this study. Among 251 patients, there were 108 (43%) meningitis patients including 73 (67.7%) healthy ones, 74 (29.5%) pulmonary infected patients including 49 (66.2%) healthy ones, and 69 (27.5%) patients with both neural and pulmonary infection including 38 (55.1%) immunocompetent ones. One hundred and forty-three (74 plus 69) patients with pulmonary cryptococcosis and 177 (108 plus 69) patients with cryptococcal meningitis were compared with controls, respectively. Three samples from 143 pulmonary infected patients failed in genotyping. There was a significant difference between 86 immunocompetent pulmonary infected patients and controls in the overdominant model (C/T vs. T/T + C/C; OR, 0.59; 95% CI, 0.37-0.94; P,.026). Similar but not significant difference was found between the overall pulmonary infected patients and the controls in the overdominant model (OR, 0.77; 95% CI, 0.52-1.12; P,.17). No such difference was found between controls and patients with cryptococcal meningitis. Our study firstly showed a genetic association between Dectin-2 and pulmonary cryptococcosis.