INTERACTION OF HEMOGLOBIN WITH IONS - QUANTITATIVE DESCRIPTION OF STATE OF MAGNESIUM, ADENOSINE 5'-TRIPHOSPHATE, 2,3-BISPHOSPHOGLYCERATE, AND HUMAN HEMOGLOBIN UNDER SIMULATED INTRACELLULAR CONDITIONS
INTERACTION OF HEMOGLOBIN WITH IONS - QUANTITATIVE DESCRIPTION OF STATE OF MAGNESIUM, ADENOSINE 5'-TRIPHOSPHATE, 2,3-BISPHOSPHOGLYCERATE, AND HUMAN HEMOGLOBIN UNDER SIMULATED INTRACELLULAR CONDITIONS
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DOI：
10.1111/j.1432-1033.1973.tb03091.x
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发表时间：
1973-01-01
期刊：
影响因子：
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通讯作者：
RAPOPORT, SM
中科院分区：
文献类型：
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作者：
GERBER, G;BERGER, H;RAPOPORT, SM
根据相关复合物的关联常数，计算了正常范围内含氧和缺氧人红细胞的ATP、2,3‐双磷酸甘油（P2‐甘油）和Mg2+的细胞内分布。数据表明，大约20%的ATP在氧化和脱氧细胞中结合，而在这些条件下p2 -甘油的结合率分别为39%和73%。血红蛋白的完全脱氧使游离Mg2+浓度从0.7 mM增加到1.1 mM。计算表明，在血红蛋白脱氧过程中，己糖激酶与激活剂Mg2+浓度的增加和抑制剂p2 -甘油的下降发生反应，其活性升高，这与完整红细胞的实验数据相对应。根据p2 -甘油的游离浓度和双磷酸甘油变异酶的动力学常数估计，脱氧细胞的p2 -甘油形成速率比氧化细胞高2.5倍。对包括碳酸氢盐在内的其他阴离子可能影响的评估表明，ATP、P2‐甘油酸和Mg2+的种类分布变化不到20%。与所附论文中的数据一起，本文给出的结果表明，常数和估计值对细胞内条件近似有效。
The intracellular distribution of ATP, 2,3‐bisphosphoglycerate(P2‐glycerate) and Mg2+was calculated for the oxygenated and deoxygenated human erythrocyte for the normal range and that of pathophysiological variations based on the association constants of the relevant complexes.The data indicate that about 20% of ATP is bound both in the oxygenated and deoxygenated cells, while 39 and 73% ofP2‐glycerate is bound under these conditions. An increase of the free Mg2+concentration from 0.7 to 1.1 mM is produced by complete deoxygenation of haemoglobin.The calculations would indicate that during deoxygenation of haemoglobin the hexokinase reacts to the increased concentration of the activator Mg2+and the decline of the inhibitorP2‐glycerate with an elevation of its activity which corresponds to experimental data on intact erythrocytes.TheP2‐glycerate formation rate in deoxygenated cells is stimulated about 2.5 times in comparison to oxygenated cells as estimated from the free concentration ofP2‐glycerate and the kinetic constants of bisphosphoglycerate mutase.An assessment of the possible influence of other anions including bicarbonate shows that the distribution of the species of ATP,P2‐glycerate and Mg2+are changed by less than 20%.Together with the data presented in the accompanying paper, the results given here indicate that the constants and estimates are approximately valid for intracellular conditions.