Monitoring food preference in Drosophila by oligonucleotide tagging
Monitoring food preference in Drosophila by oligonucleotide tagging
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DOI：
10.1073/pnas.1716880115
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发表时间：
2018-09-04
影响因子：
11.1
通讯作者：
Atkinson, Nigel S.
中科院分区：
文献类型：
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作者：
Park, Annie;Tran, Tracy;Atkinson, Nigel S.
黑腹果蝇是研究食欲和厌恶行为神经发生基础的重要模式生物。然而，一些用于测定食物偏好的方法需要或导致饥饿。这对苍蝇乙醇研究来说可能是个问题，因为很难将乙醇的热量偏好与药物的药理学偏好分开。我们设计了BARCODE，这是一种不依赖于饥饿的检测方法，它使用痕量的寡核苷酸标签来区别标记食物类型。在BARCODE中，苍蝇随意进食，通过寡核苷酸的qPCR监测相对食物偏好。摄入的低聚物在苍蝇内的持续性记录了苍蝇数天的进食史。使用条形码，我们确定了对乙醇的性别二态偏好。雌性喜欢含乙醇的食物，而雄性则避免食用。此外，基因雌性化的雄性蘑菇体叶诱导了对乙醇的偏好。此外，我们还证明了当动物有两种以上的食物选择时，BARCODE可用于多重饮食测量。
Drosophila melanogaster is a powerful model organism for dissecting the neurogenetic basis of appetitive and aversive behaviors. However, some methods used to assay food preference require or cause starvation. This can be problematic for fly ethanol research because it can be difficult to dissociate caloric preference for ethanol from pharmacological preference for the drug. We designed BARCODE, a starvation-independent assay that uses trace levels of oligonucleotide tags to differentially mark food types. In BARCODE, flies feed ad libitum, and relative food preference is monitored by qPCR of the oligonucleotides. Persistence of the ingested oligomers within the fly records the feeding history of the fly over several days. Using BARCODE, we identified a sexually dimorphic preference for ethanol. Females are attracted to ethanol-laden foods, whereas males avoid consuming it. Furthermore, genetically feminizing male mushroom body lobes induces preference for ethanol. In addition, we demonstrate that BARCODE can be used for multiplex diet measurements when animals are presented with more than two food choices.