Datasets of microarray analysis to identify Gpr137b-dependent interleukin-4-responsive genes in the mouse macrophage cell line RAW264
Datasets of microarray analysis to identify Gpr137b-dependent interleukin-4-responsive genes in the mouse macrophage cell line RAW264
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用于鉴定小鼠巨噬细胞系 RAW264 中 Gpr137b 依赖性白细胞介素 4 反应基因的微阵列分析数据集
DOI：
10.1016/j.dib.2019.01.017
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发表时间：
2019
期刊：
影响因子：
1.2
通讯作者：
Ishibashi Osamu
中科院分区：
文献类型：
--
作者：
Islam Zohirul;Horikawa Aya;Inui Takashi;Ishibashi Osamu
巨噬细胞主要分为M1和M2两种亚型，它们根据微环境的不同表现出不同的表型。我们最近证实Gpr137bis在RAW264巨噬细胞中大量表达，“Gpr137bis是与M2巨噬细胞极化相关的孤儿g蛋白偶联受体”（Islam等，in press） bbb。尽管最近的研究表明，g蛋白偶联受体（gpcr）与M1/M2巨噬细胞极化有关（“g蛋白偶联胆红酸受体1 （GPBAR1, TGR5）激动剂减少促炎细胞因子的产生并稳定替代性巨噬细胞表型”（Hogenauer等，2014）[3]，“白三烯B4促进小鼠湿型AMD模型中的新生血管和巨噬细胞募集”（Sasaki等，2018）[3]）。关于gpcr介导的巨噬细胞极化的信息仍然有限。这促使我们使用CRISPR/Cas9基因组编辑系统生成ategpr137b敲除（KO） RAW264克隆，以阐明gpr137bin白素(IL)-4诱导的M2巨噬细胞极化的功能（Islam et al., in press）[1]。在这里，我们展示了微阵列分析的数据集，以鉴定RAW264细胞中依赖gpr137b的il -4应答基因。原始微阵列数据可在Gene Expression Omnibus数据库（https://www.ncbi.nlm.nih.gov/geo/）中获得，登录号为GSE117578， https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE117578。
Macrophages are classified mainly into two subtypes, M1 and M2, which exhibit distinct phenotypes, based on their microenvironment. We have recently demonstrated thatGpr137bis abundantly expressed in RAW264 macrophages, “Gpr137bis an orphan G-protein-coupled receptor associated with M2 macrophage polarization” (Islam et al., in press) [1]. Although recent studies have suggested that G-protein-coupled receptors (GPCRs) are associated with M1/M2 macrophage polarization (“G-protein-coupled bile acid receptor 1 (GPBAR1, TGR5) agonists reduce the production of proinflammatory cytokines and stabilize the alternative macrophage phenotype” (Hogenauer et al., 2014) [2], “Leukotriene B4 promotes neovascularization and macrophage recruitment in murine wet-type AMD models” (Sasaki et al., 2018) [3]), available information about GPCR-mediated macrophage polarization is still limited. This prompted us to generateGpr137b-knockout (KO) RAW264 clones using the CRISPR/Cas9 genome editing system to elucidate the function ofGpr137bin interleukin (IL)-4-induced M2 macrophage polarization (Islam et al., in press) [1].Here we present the datasets of a microarray analysis to identifyGpr137b-dependent IL-4-responsive genes in RAW264 cells. The raw microarray data are available in the Gene Expression Omnibus database (https://www.ncbi.nlm.nih.gov/geo/) under the accession number GSE117578, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE117578.