Mycobacterium avium subsp paratuberculosis in scavenging mammals in Wisconsin
Mycobacterium avium subsp paratuberculosis in scavenging mammals in Wisconsin
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DOI：
10.7589/0090-3558-43.2.302
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发表时间：
2007-04-01
影响因子：
1.3
通讯作者：
Ellingson, Jay L. E.
中科院分区：
文献类型：
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作者：
Anderson, Jennifer L.;Meece, Jennifer K.;Ellingson, Jay L. E.
鸟分枝杆菌亚种的存在。非反胃野生动物中的副结核(MAP)引起了人们对这些物种在约翰氏病传播中所起作用的质疑。在这项研究中，我们测试了六种不同种类的食腐哺乳动物的212只动物的472个组织。2003年9月至2004年5月，所有动物都是从威斯康星州中南部戴恩县和爱荷华县210平方英里的范围内采集的，并测试了地图的存在。我们在212只食腐哺乳动物中有81只(38%)检测到MAP特异性DNA，在472只组织中有98只(21%)检测到MAP特异性DNA；从一只郊狼的回肠和淋巴结组织中培养出有活力的MAP。尽管在本研究中分离到的有活力的MAP数量很少，但我们的数据增加了越来越多的证据，证明MAP在非反刍动物物种中传播和感染的可能性，并提供了物种间传播的可能证据。显然，非反刍动物的高暴露提供了MAP对野生动物物种溢出的潜在证据，并提出了溢出到家养和野生反刍动物的问题。这些结果证明了了解野生动物物种在制定家畜约翰氏病管理策略中的作用的重要性。
The presence of Mycobacterium avium subsp. paratuberculosis (MAP) in non-ruminant wildlife has raised questions regarding the role of these species in Johne's disease transmission. In this study we tested 472 tissues from 212 animals of six different species of scavenging mammals. All animals were taken from within a 210-square-mile area in Dane and Iowa counties of south central Wisconsin from September to May in 2003-04 and tested for the presence of MAP. We detected MAP-specific DNA in 81 of 212 (38%) scavenging mammals, in 98 of the 472 (21%) tissues; viable MAP was cultured from one coyote's ileum and lymph node tissue. Despite the low numbers of viable MAP isolated in this study, our data adds to the increasing evidence demonstrating the potential for transmission and infection of MAP in nonruminant species and provides possible evidence of interspecies transmission. The apparently high exposure of nonruminant wildlife provides potential evidence of a spillover of MAP to wildlife species and raises the question of spillback to domestic and wild ruminants. These results demonstrate the importance of understanding the role of wildlife species in developing management strategies for Johne's disease in domestic livestock.