Effects of glucocorticoids on fetal rat bone collagen synthesis in vitro.
Effects of glucocorticoids on fetal rat bone collagen synthesis in vitro.
复制标题
糖皮质激素对体外胎鼠骨胶原合成的影响。
作者：
J. Dietrich;E. Canalis;D. Maina;L. Raisz
在器官培养中检测糖皮质激素对骨胶原合成的影响。胎鼠颅骨孵育与[3 H]脯氨酸在最后2小时的文化; [3 H]脯氨酸掺入胶原酶消化（CDP）和非胶原蛋白（NCP）使用纯化的细菌胶原酶测定。皮质醇（0.03-3 μM）在24小时后增加标记物的掺入，尽管CDP比NCP受影响更大。皮质醇没有改变胶原蛋白的合成新鲜骨培养3小时。在高浓度和低浓度的未标记脯氨酸中观察到刺激作用，并且与[3 H]胸苷掺入DNA或[3 H]尿苷掺入RNA的增加无关;标记胶原从骨释放到培养基不受皮质醇的影响。地塞米松（9α-氟-11 β，17 α，21-三羟基-16 α-甲基孕甾-1，4-二烯-3，20-二酮; 1 nM）、皮质酮和皮质醇酮（17α，21-二羟基-4-孕甾烯-3，20-二酮; 0.1 μM）24 h后增加胶原合成;表皮质醇（11α，17 α，21-三氢-1，4-二烯-3，20-二酮; 0.1 μM）24 h后增加胶原合成。
Effects of glucocorticoids on bone collagen synthesis were examined in organ cultures. Fetal rat calvaria were incubated with [3H]proline during the last 2 h of culture; [3H] proline incorporation into collagenase-digestible (CDP) and noncollagen protein (NCP) was measured using purified bacterial collagenase. Cortisol (0.03-3 μM) increased incorporation of label after 24 h, although CDP was affected more than NCP. Cortisol did not alter collagen synthesis in freshly explanted bones cultured for 3 h. The stimulatory effect was seen at high and low concentrations of unlabeled proline and was not associated with increased incorporation of [3H]thymidine into DNA or [3H]uridine into RNA; release of labeled collagen from bone to medium was unaffected by cortisol. Dexamethasone (9α-fluoro- 11β,17α,21-trihydroxy- 16α-methylpregna- l,4-diene-3,20-dione; 1 nM), corticosterone, and cortexolone (17α,21-dihydroxy-4-pregnene- 3,20-dione; 0.1 μM) increased collagen synthesis after 24 h; epicortisol (11α,17α,21-trihydro...