Measurements of age-related changes of physiological processes that predict lifespan of Caenorhabditis elegans
Measurements of age-related changes of physiological processes that predict lifespan of Caenorhabditis elegans
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DOI：
10.1073/pnas.0400848101
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发表时间：
2004-05-25
影响因子：
11.1
通讯作者：
Kornfeld, K
中科院分区：
文献类型：
--
作者：
Huang, C;Xiong, CJ;Kornfeld, K
衰老的特征是许多组织中的进行性退行性变化。为了阐明秀丽隐杆线虫退行性变化之间的关系，我们开发了定量测量年龄相关变化的方法，并通过纵向研究分析了这些变化之间的相关性。咽泵功能和身体运动功能的增龄性下降与彼此和寿命呈正相关。这些研究结果表明，咽泵和身体运动的下降导致生存概率下降，或者共同的调节系统介导了咽泵，身体运动和生存概率的下降。此外，这些过程的测量可用于预测寿命和检测过早老化。生理过程的下降是在daf-2，age-1，daf-16，eat-2和eat-1突变体中测量的，这些突变体改变了寿命。每个突变株显示一个或多个与年龄相关的下降的变化，但与年龄相关的变化之间的相关性与WT相似。这些测量结果被用来生成一个描述衰老过程的四个阶段的系统，并用于分析遗传和环境对衰老的影响。
Aging is characterized by progressive, degenerative changes in many tissues. To elucidate the relationships among degenerative changes in Caenorhabditis elegans, we developed methods to measure age-related changes quantitatively and analyzed correlations among these changes by using a longitudinal study. The age-related declines of pharyngeal pumping and body movement were positively correlated with each other and lifespan. These findings suggest that the declines of pharyngeal pumping and body movement cause a decline in survival probability or that a shared regulatory system mediates the declines in pharyngeal pumping, body movement, and survival probability. Furthermore, measurements of these processes can be used to predict lifespan and detect premature aging. The declines of physiological processes were measured in daf-2, age-1, daf-16, eat-2, and clk-1 mutants that have altered lifespans. Each mutant strain displayed changes in one or more age-related declines, but the correlations among age-related changes were similar to WT. These measurements were used to generate a system of four stages that describes the aging process and is useful for the analysis of genetic and environmental effects on aging.