Discovery and characterization of two Isoforms of moronecidin, a novel antimicrobial peptide from hybrid striped bass
Discovery and characterization of two Isoforms of moronecidin, a novel antimicrobial peptide from hybrid striped bass
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DOI：
10.1074/jbc.m109173200
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发表时间：
2002-02-15
影响因子：
4.8
通讯作者：
Bulet, P
中科院分区：
文献类型：
--
作者：
Lauth, X;Shike, H;Bulet, P
我们从杂交条纹鲈鱼的皮肤和鳃中分离到一种新的22个残基，C-末端酰胺化的抗菌肽moronecidin。两种同种型，仅一个氨基酸不同，来自每个亲本物种，白色鲈鱼（Morone chrysops）和条纹鲈鱼（Morone saxatilis）。测定每种亚型的分子量（2543和2571 Da）、氨基酸序列（FFHHIFRGIVHVGKTIH（K/R）LVTGT）、cDNA和基因组DNA序列。预测的79个残基的moronecidin prepropeptide由三个结构域组成：信号肽（22个氨基酸），成熟肽（22个氨基酸），和C-末端prodomain（35个氨基酸）。合成的酰胺化的白色低音moronecidin表现出广谱抗微生物活性，在高盐浓度下保留。通过圆二色性光谱证实了a-螺旋结构。moronecidin基因由三个内含子和四个外显子组成。肽序列和基因结构与比目鱼抗菌肽pleurocidin相似。在转录起始位点的5'端区域发现了一个TATA盒和几个转录因子的一致结合基序。采用动态逆转录-聚合酶链反应（RT-PCR）检测了Moronecidin基因在鳃、皮肤、肠、脾、前肾和血细胞中的表达。因此，moronecidin是一种新的α-螺旋，广谱抗菌肽分离的皮肤和鳃的杂交条纹鲈鱼。
We isolated a novel 22-residue, C-terminally amidated antimicrobial peptide, moronecidin, from the skin and gill of hybrid striped bass. Two isoforms, differing by only one amino acid, are derived from each parental species, white bass (Morone chrysops) and striped bass (Morone saxatilis). Molecular masses (2543 and 2571 Da), amino acid sequences (FFHHIFRGIVHVGKTIH(K/R) LVTGT), cDNA, and genomic DNA sequences were determined for each isoform. A predicted 79-residue moronecidin prepropeptide consists of three domains: a signal peptide (22 amino acids), a mature peptide (22 amino acids), and a C-terminal prodomain (35 amino acids). The synthetic, amidated white bass moronecidin exhibited broad spectrum antimicrobial activity that was retained at high salt concentration. An a-helical structure was confirmed by circular dichroism spectroscopy. The moronecidin gene consists of three introns and four exons. Peptide sequence and gene organization were similar to pleurocidin, an antimicrobial peptide from winter flounder. A TATA box and several consensus-binding motifs for transcription factors were found in the region 5' to the transcriptional start site. Moronecidin gene expression was detected in gill, skin, intestine, spleen, anterior kidney, and blood cells by kinetic reverse transcription (RT)-PCR. Thus, moronecidin is a new a-helical, broad spectrum antimicrobial peptide isolated from the skin and gills of hybrid striped bass.