Expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and peroxiredoxin 6 (Prdx6) proteins in healthy and pathologic placentas of human and rat
Expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and peroxiredoxin 6 (Prdx6) proteins in healthy and pathologic placentas of human and rat
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DOI：
10.1016/j.acthis.2014.07.012
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发表时间：
2014-01-01
影响因子：
2.5
通讯作者：
Ustunel, Ismail
中科院分区：
文献类型：
--
作者：
Acar, Nuray;Soylu, Hakan;Ustunel, Ismail
子痫前期(PE)与胎儿宫内生长受限(IUGR)和氧化应激(OS)之间存在一定的关系。由于这样的妊娠经历了OS，我们的目的是利用免疫组织化学和免疫印迹技术检测在人类健康、IUGR、PE以及大鼠健康和IUGR胎盘中具有抗氧化酶调节作用的转录因子核因子红系2相关因子2(NRF2)和抗氧化酶Prdx6(Prdx6)的分布模式和表达水平。人和大鼠IUGR组胎盘中Nrf2和Prdx6免疫反应分别弱于人和大鼠对照组胎盘。对照组和IUGR组胎盘迷路滋养细胞、蜕膜、巨细胞、糖原和胎儿血管内皮细胞表达NRF2和Prdx6。对照组、IUGR组和PE组胎盘蜕膜、合体滋养层细胞、绒毛基质细胞和血管内皮细胞表达NRF2和Prdx6。应用于大鼠和人胎盘的Nrf2和Prdx6免疫印迹结果与大鼠和人胎盘的Nrf2和Prdx6免疫组织化学观察结果一致。人和大鼠IUGR组胎盘中Nrf2和Prdx6蛋白表达下调可能导致OS的形成，OS可能损害细胞滋养层细胞的增殖和侵袭。(C)2014年爱思唯尔股份有限公司。版权所有。
A relationship has been shown between preeclampsia (PE) and intrauterine growth restriction (IUGR) and oxidative stress (OS). Since such pregnancies experience OS, we aimed to detect the distribution pattern and expression levels of a transcription factor, Nuclear factor erythroid 2-related factor-2 (Nrf2) that has a role in the regulation of antioxidant enzymes, and peroxiredoxin 6 (Prdx6) an antioxidant enzyme, in human healthy, IUGR, PE and in groups of rat healthy and IUGR placentas using immunohistochemistry and Western blotting. Both Nrf2 and Prdx6 immunoreactivities were weaker in human and rat IUGR group placentas compared to human and rat control group placentas, respectively. Nrf2 and Prdx6 were immunostained in labyrinth trophoblasts, decidua, giant, glycogen and fetal vessel endothelial cells in rat control and IUGR group placentas. Nrf2 and Prdx6 immunoreactivities were seen in the decidua, syncytiotrophoblasts, villous stromal cells, and vascular endothelium in human control, IUGR and PE group placentas. Results of Nrf2 and Prdx6 Western blotting applied for rat and human placentas were compatible with the results of Nrf2 and Prdx6 immunohistochemical observations with regard to rat and human placentas. Down-regulation of Nrf2 and Prdx6 proteins in human and rat IUGR group placentas may have led to the formation of OS which may have impaired proliferation and invasion of cytotrophoblasts. (C) 2014 Elsevier GmbH. All rights reserved.