PLEODORINA-JAPONICA SP-NOV (VOLVOCALES, CHLOROPHYTA) WITH BACTERIA-LIKE ENDOSYMBIONTS
PLEODORINA-JAPONICA SP-NOV (VOLVOCALES, CHLOROPHYTA) WITH BACTERIA-LIKE ENDOSYMBIONTS
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DOI：
10.2216/i0031-8884-28-2-252.1
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发表时间：
1989-06-01
期刊：
影响因子：
1.6
通讯作者：
KUROIWA, T
中科院分区：
文献类型：
--
作者：
NOZAKI, H;KUROIWA, H;KUROIWA, T
利用无性系培养技术对日本野崎多萝菇的形态、繁殖和分类进行了研究。该分类群与其他多角藻属和假多角藻属的区别在于其独特的营养菌落，其中只有20-27%的细胞是体细胞和前体细胞，胶质基质不形成单独的鞘。在多萝莲中首次观察到合子萌发。单个双鞭毛细胞从受精卵壁上移出。此外，通过4′-6-二氨基-2-苯基吲哚（DAPI）染色和电镜观察发现，在体细胞和生殖细胞细胞质以及雌雄配子和合子中存在丰富的细菌样内共生体。内共生菌呈刚性棒状，面积1.5 ~ 1.9亩。经DAPI染色后，在聚光显微镜下发出强烈的蓝白色荧光，周围有电子透明区。讨论了多菇属（Pleodorina）与尤多菇属（Eudorina）的属界划分和种分类，并对多菇属（Pleodorina） 4种、加利福尼亚多菇属（P. californica Shaw）、球形多菇属（P. sphaerica lyengar）、印度多菇属（P. Iyengar） Nozaki comb进行了讨论。11 .和P. japonica，被识别。
The morphology, reproduction and taxonomy of Pleodorina japonica Nozaki sp. nov. collected in Japan were studied using clonal cultures. This taxon is distinguished from the other species of Pleodorina and Eudorina by its unique vegetative colonies, in which only 20-27% of the cells are somatic and anterior, and in which the gelatinous matrix does not form individual sheaths. Zygote germination was observed for the first time in Pleodorina. A single biflagellate gone-cell moved out from the zygote wall. In addition, abundant bacteria-like endosymbionts in the cytoplasm of somatic and reproductive cells as well as in the male and female gametes and zygotes were revealed by means of 4''-6-diamidino-2-phenylindole (DAPI) staining and electron microscopy. The endosymbiont was rigidly rod-shaped, measured 1.5-1.9 .mu.m long and emitted strong blue-white fluorescence under the epifluorescence microscope after DAPI staining, and it was surrounded by electron transparent areas. The generic delimitation between Pleodorina and Eudorina as well as taxonomy of species of Pleodorina are discussed, and four species of the genus Pleodorina, P. californica Shaw, P. sphaerica lyengar, P. indica (Iyengar) Nozaki comb. nov. and P. japonica, are recognized.