ECHS1 acts as a novel HBsAg-binding protein enhancing apoptosis through the mitochondrial pathway in HepG2 cells
ECHS1 acts as a novel HBsAg-binding protein enhancing apoptosis through the mitochondrial pathway in HepG2 cells
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ECHS1 作为一种新型 HBsAg 结合蛋白，通过线粒体途径增强 HepG2 细胞的细胞凋亡
DOI：
10.1016/j.canlet.2012.11.030
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发表时间：
2013-03-01
期刊：
影响因子：
9.7
通讯作者：
Ren, Jian-Lin
中科院分区：
文献类型：
--
作者：
Xiao, Chuan-Xing;Yang, Xiao-Ning;Ren, Jian-Lin
本研究旨在证实ECHS1作为乙肝表面抗原(HBs)结合蛋白的作用，并探讨其在肝细胞癌发生发展中的作用。我们的结果表明，外源性和内源性ECHS1蛋白都能与HBs结合，并在体外共定位于细胞质中。HBs和ECHS1的共存促进了HepG2细胞的凋亡，影响了ECHS1在线粒体中的定位，并通过降低线粒体膜电位而诱导细胞凋亡。这些发现提示ECHS1可能成为治疗乙肝相关性肝炎或肝细胞癌的潜在靶点。(C)2012爱思唯尔爱尔兰有限公司。保留所有权利。
We aimed to confirm the role of ECHS1 as a binding protein of HBsAg (HBs) and investigate its function during the development of hepatocellular carcinoma (HCC). Our results show that both exogenous and endogenous ECHS1 proteins bind to HBs and co-localize in the cytoplasm in vitro. The coexistence of HBs and ECHS1 enhances HepG2 cell apoptosis, affects ECHS1 localization in the mitochondria and induces apoptosis by decreasing the mitochondrial membrane potential (MMP). These findings suggest that ECHS1 may be applied as a potential therapeutic target during the treatment of HBV-related hepatitis or HCC. (C) 2012 Elsevier Ireland Ltd. All rights reserved.