γ-Glutamyl transpeptidase has a role in the persistent colonization of the avian gut by Campylobacter jejuni
γ-Glutamyl transpeptidase has a role in the persistent colonization of the avian gut by Campylobacter jejuni
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DOI：
10.1016/j.micpath.2007.05.007
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发表时间：
2007-11-01
影响因子：
3.8
通讯作者：
Newell, Diane G.
中科院分区：
文献类型：
--
作者：
Barnes, H. A.;Bagnall, Mary C.;Newell, Diane G.
The contribution of gamma-glutamyl transpeptidase (GGT) to Campylobacter jejuni virulence and colonization of the avian gut has been investigated. The presence of the ggt gene in C jejuni strains directly correlated with the expression of GGT activity as measured by cleavage and transfer of the gamma-glutamyl moiety. Inactivation of the monocistronic ggt gene in C jejuni strain 81116 resulted in isogenic mutants with undetectable GGT activity; nevertheless, these mutants grew normally in vitro. However, the mutants had increased motility, a 5.4-fold higher invasion efficiency into INT407 cells in vitro and increased resistance to hydrogen peroxide stress. Moreover, the apoptosis-inducing activity of the ggt mutant was significantly lower than that of the parental strain. In vivo studies showed that., although GGT activity was not required for initial colonization of 1-day-old chicks, the enzyme was required for persistant colonization of the avian gut. (C) 2007 Elsevier Ltd. All rights reserved.
