Plant metabolomics-meeting the analytical challenges of comprehensive metabolite analysis
Plant metabolomics-meeting the analytical challenges of comprehensive metabolite analysis
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DOI：
10.1093/bfgp/elp053
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发表时间：
2010-03-01
影响因子：
4
通讯作者：
Hegeman, Adrian D.
中科院分区：
文献类型：
--
作者：
Hegeman, Adrian D.
植物代谢组学得益于一系列丰富的预先存在的方法学方法和生物分析知识，用于表征许多化学上不同类别的代谢物。虽然该领域已经推动了代谢物提取，分馏和检测的无偏见和普遍适用的策略的实施，但在基本活动中仍然存在重大挑战，如化合物鉴定和定量。这篇综述介绍了代谢组学的术语、方法和资源，并讨论了分析的局限性。本文综述了稳定同位素稀释法和稳定同位素代谢标记法在代谢物鉴定和定量中的应用进展。新的策略，以解决空间分布的代谢物，通过质谱为基础的成像也进行了审查。
Plant metabolomics has benefited from a rich array of pre-existing methodological approaches and bioanalytical knowledge for the characterization of the many chemically diverse classes of metabolites. While the field has pushed the implementation of unbiased and generally applicable strategies for metabolite extraction, fractionation and detection, significant challenges in fundamental activities such as compound identification and quantification still exist. This review provides an introduction to metabolomics terminology, methods and resources and discusses analytical limitations. Progress in the application of stable isotope dilution and stable isotopic metabolic labeling to metabolite identification and quantification are discussed. New strategies to address spatial distribution of metabolites via mass spectrometry-based imaging are also reviewed.