Under-nutrition reduces spermatogenic efficiency and sperm velocity, and increases sperm DNA damage in sexually mature male sheep
Under-nutrition reduces spermatogenic efficiency and sperm velocity, and increases sperm DNA damage in sexually mature male sheep
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DOI：
10.1016/j.anireprosci.2014.07.014
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发表时间：
2014-10-01
影响因子：
2.2
通讯作者：
Martin, Graeme B.
中科院分区：
文献类型：
--
作者：
Guan, Yongjuan;Malecki, Irek A.;Martin, Graeme B.
我们测试了在营养诱导的睾丸质量变化过程中，成熟雄性绵羊的精子质量是否会受到影响。饲喂增加、维持或减少体、睾丸质量的饲粮（每组8只），试验期65 d。在第56天和第63天采集的精液中，饲喂不足的公羊每次射精的精子数量低于饲喂充足的公羊（P < 0.05），精子速度（计算机辅助精液分析）低于饲喂充足和维持喂养的公羊（P < 0.05）。精子染色质结构分析显示，营养不良的公羊精子DNA损伤程度高于营养良好的公羊（P < 0.05）。精子DNA损伤量与阴囊周长（r= -0.6, P < 0.05）、进行性活动精子百分比（r= -0.8, P < 0.01）、活动精子百分比（r= -0.6, P < 0.05）、每克睾丸精子数（r= -0.55, P < 0.05）呈负相关。在第65天收集的睾丸组织中，营养不良的公羊每克睾丸的精子数量少于其他两组公羊（P < 0.001）。我们得出的结论是，在成年公羊中，摄食不足降低了生精效率，这种反应与精子质量的降低有关。(C) 2014 Elsevier B.V.版权所有
We tested whether the quality of spermatozoa from mature male sheep would be affected during nutrition-induced changes in testicular mass. Merino rams were fed for 65 days with diets that increased, maintained or decreased body and testis mass (n = 8 per group). In semen collected on Days 56 and 63, underfed rams had less sperms per ejaculate than wellfed rams (P < 0.05) and a lower sperm velocity (computer-assisted semen analysis) than well-fed or maintenance-fed rams (P < 0.05). Sperm chromatin structure assay revealed more sperm DNA damage in underfed rams than in well-fed rams (P < 0.05). The amount of sperm DNA damage was inversely correlated with change in scrotal circumference (r= -0.6, P < 0.05), the percentages of progressive motile sperm (r= -0.8; P < 0.01) and motile sperm (r = -0.6, P < 0.05), and the numbers of sperms per gram of testis (r= -0.55, P < 0.05). In testicular tissue collected on Day 65, underfed rams had fewer sperm per gram of testis than rams in the other two groups (P < 0.001). We conclude that, in adult rams, underfeeding reduces spermatogenic efficiency and that this response is associated with a reduction in spermatozoal quality. (C) 2014 Elsevier B.V. All rights reserved.