SHED promote angiogenesis in stem cell-mediated dental pulp regeneration
SHED promote angiogenesis in stem cell-mediated dental pulp regeneration
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SHED促进干细胞介导的牙髓再生中的血管生成
DOI：
10.1016/j.bbrc.2020.06.151
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发表时间：
2020-09-03
影响因子：
3.1
通讯作者：
Jin, Yan
中科院分区：
文献类型：
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作者：
Guo, Hao;Zhao, Wanmin;Jin, Yan
牙髓在维持牙齿的内环境平衡中起着不可缺少的作用。牙髓坏死往往会导致牙齿营养不足，牙根发育异常，从而导致牙齿变色、断裂甚至脱落。我们前期的研究表明自体SHED移植可以再生功能性牙髓。然而，植入的SHED参与牙髓再生的具体机制尚不清楚。在本研究中，我们将SHED植入猪牙髓再生模型，评估其再生效果，并确定SHED是否促进了再生牙髓的血管生成。首先，我们在体内验证了异种SHED具有再生宿主牙髓组织的能力。然后我们发现再生髓内的血管系统。此外，从再生牙髓中分离出干细胞，干细胞具有良好的多分化特性，促进了牙髓再生过程中的血管生成，这些结果表明SHED促进了干细胞介导的牙髓再生中的血管生成。(C) 2020爱思唯尔公司版权所有。
Dental pulp, plays an indispensable role in maintaining homeostasis of the tooth. Pulp necrosis always causes tooth nutrition deficiency and abnormal root development, which leads to tooth discoloration, fracture or even loss. Our previous study showed implantation of autologous SHED could regenerate functional dental pulp. However, the detailed mechanism of the implanted SHED participating in dental pulp regeneration remains unknown. In this study, we implanted SHED in a porcine dental pulp regeneration model to evaluate the regenerative effect and identify whether SHED promoted angiogenesis in regenerated dental pulp. Firstly we verified that xenogenous SHED had the ability to regenerated pulp tissue of host in vivo. Then we found the vasculature in regenerated pulp originated from implanted SHED. In addition, stem cells were isolated from regenerated dental pulp, which exhibited good multi-differentiation properties and promoted angiogenesis in pulp regeneration process and these results demonstrated that SHED promoted angiogenesis in stem cell-mediated dental pulp regeneration. (C) 2020 Elsevier Inc. All rights reserved.