Cytotoxic and apoptotic effects of six herbal plants against the human hepatocarcinoma (HepG2) cell line.
Cytotoxic and apoptotic effects of six herbal plants against the human hepatocarcinoma (HepG2) cell line.
复制标题
六种草药植物对人肝癌（HEPG2）细胞系的细胞毒性和凋亡作用。
DOI：
10.1186/1749-8546-6-39
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发表时间：
2011-10-31
期刊：
影响因子：
4.9
通讯作者：
Thitimetharoch T
中科院分区：
文献类型：
--
作者：
Machana S;Weerapreeyakul N;Barusrux S;Nonpunya A;Sripanidkulchai B;Thitimetharoch T
研究了来自泰国的6种植物对人肝癌（HepG2）的细胞毒性和诱导凋亡的作用，并与正常的非洲绿猴肾上皮细胞系进行了比较。用中性红法测定6种植物乙醇-水粗提物暴露于细胞24小时后的细胞毒性。采用二氨基苯基吲哚染色法检测HepG2细胞的凋亡诱导作用。琼脂糖凝胶电泳分析细胞凋亡的DNA片段。用4-（4′-硝基苄基）吡啶法测定了DNA损伤的烷基化程度。对HepG2细胞株的选择性最高（选择性指数为9.6），IC50值为52.0±5.8 μg/ml（平均值±标准差）。铜钛提取物具有细胞毒性，IC50值为55.7±8.1 μg/ml。金针桃、东洋枝、金针桃和砂仁粗提物具有细胞毒性，IC50值在100 ~ 500 μg/ml之间。各粗提物均表现出不同的体外烷基化能力。三种植物的提取物均引起细胞核形态变化和DNA阶梯。三种提取物均可诱导细胞凋亡。在这3种植物中，kesiya具有最强的抗癌活性，对HepG2细胞具有特异性的选择性。
Six plants from Thailand were evaluated for their cytotoxicity and apoptosis induction in human hepatocarcinoma (HepG2) as compared to normal African green monkey kidney epithelial cell lines. Ethanol-water crude extracts of the six plants were tested with neutral red assay for their cytotoxicity after 24 hours of exposure to the cells. Apoptotic induction was tested in the HepG2 cells with diamidino-2-phenylindole staining. DNA fragmentation, indicative of apoptosis, was analyzed with agarose gel electrophoresis. Alkylation, indicative of DNA damage, was also evaluated in vitro by 4-(4'-nitrobenzyl) pyridine assay. The extract of Pinus kesiya showed the highest selectivity (selectivity index = 9.6) and potent cytotoxicity in the HepG2 cell line, with an IC50 value of 52.0 ± 5.8 μg/ml (mean ± standard deviation). Extract of Catimbium speciosum exerted cytotoxicity with an IC50 value of 55.7 ± 8.1 μg/ml. Crude extracts from Glochidion daltonii, Cladogynos orientalis, Acorus tatarinowii and Amomum villosum exhibited cytotoxicity with IC50 values ranging 100-500 μg/ml. All crude extracts showed different alkylating abilities in vitro. Extracts of P. kesiya, C. speciosum and C. orientalis caused nuclei morphological changes and DNA laddering. The extracts of C. speciosum, C. orientalis and P. kesiya induced apoptosis. Among the three plants, P. kesiya possessed the most robust anticancer activity, with specific selectivity against HepG2 cells.