The Y-Maze for Assessment of Spatial Working and Reference Memory in Mice
The Y-Maze for Assessment of Spatial Working and Reference Memory in Mice
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DOI：
10.1007/978-1-4939-8994-2_10
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发表时间：
2019-01-01
期刊：
影响因子：
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通讯作者：
Sarnyai, Zoltan
中科院分区：
文献类型：
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作者：
Kraeuter, Ann-Katrin;Guest, Paul C.;Sarnyai, Zoltan
Y-迷宫可用于评估小鼠的短期记忆。自发交替是空间工作记忆的一种测量方法，可以通过让小鼠探索迷宫的所有三个臂来评估，并且由啮齿动物探索以前未去过的区域的先天好奇心驱动。一只工作记忆完整的老鼠，其前额叶皮质功能也是完整的，它会记住之前去过的手臂，并表现出进入最近去过的手臂的倾向。空间参考记忆（以海马体为下划线）也可以通过将测试老鼠放入Y迷宫进行测试，其中一只手臂在训练期间关闭。在例如1小时的试验间隔后，小鼠应该记住它以前没有探索过哪个臂，并且应该更频繁地访问这个臂。本章描述了试验前条件、所需材料以及进行和解释这两项相关试验结果的方案。
The Y-maze can be used to assess short term memory in mice. Spontaneous alternation, a measure of spatial working memory, can be assessed by allowing mice to explore all three arms of the maze and is driven by an innate curiosity of rodents to explore previously unvisited areas. A mouse with intact working memory, and hence intact prefrontal cortical functions, will remember the arms previously visited and show a tendency to enter a less recently visited arm. Spatial reference memory, which is underlined by the hippocampus, can also be tested by placing the test mice into the Y-maze with one arm closed off during training. After an intertrial interval of for example 1 h, the mouse should remember which arm it has not explored previously and should visit this arm more often. This chapter describes the pre-test conditions, the materials required and the protocol for conducting and interpreting the results of these two related tests.