Determination of Helicobacter pylori virulence by analysis of the cag pathogenicity island isolated from Iranian patients.
Determination of Helicobacter pylori virulence by analysis of the cag pathogenicity island isolated from Iranian patients.
复制标题
DOI：
10.1016/j.dld.2009.01.010
复制
发表时间：
2009-09
期刊：
影响因子：
--
通讯作者：
Zali MR
中科院分区：
文献类型：
--
作者：
Baghaei K;Shokrzadeh L;Jafari F;Dabiri H;Yamaoka Y;Bolfion M;Zojaji H;Aslani MM;Zali MR
幽门螺杆菌（Helicobacter pylori，Hp）的cag致病岛（pylori pathogenicity island，派）是幽门螺杆菌（Helicobacter pylori，Hp）的主要毒力因子，分为cagI和cagII两部分。我们调查了伊朗人群中cag派（cagI中的cagA和cagE以及cagII中的cagT）的遗传变异与临床结局之间的相关性。共231例患者，其中胃炎182例，消化性溃疡41例，胃癌8例。采用聚合酶链反应检测cagA、cagE和cagT基因的表达，并与临床结果和胃组织学进行比较。cagA、cagE和cagT基因的检出率分别为66.7%、39.0%和30.3%。至少144株（62.3%）具有部分缺失的cag派（例如，69株（29.9%）cagA阳性，cagE和cagT阴性。cag派中的单一基因以及基因组合似乎不是预测H的有用标记。伊朗人口中的幽门螺杆菌相关疾病。伊朗菌株中的cag派的基因组序列可能与其他地理位置的基因组序列有很大不同。
The cag pathogenicity island (PAI), which can divide into two parts: cagI and cagII, is the most well-known virulence factor of Helicobacter pylori. We investigated the association between genetic variations within the cag PAI (cagA and cagE in the cagI and cagT in the cagII) and clinical outcomes in Iranian population. A total of 231 patients including 182 patients with gastritis, 41 with peptic ulcer and 8 with gastric cancer. The presences of the cagA, cagE and cagT genes were measured by polymerase chain reaction and the results were compared with clinical outcomes and gastric histology. The cagA, cagE and cagT genes were found in 154 (66.7%), 90 (39.0%) and 70 (30.3%) of clinical isolates. At least 144 (62.3%) strains possessed partially deleted cag PAI (e.g., 69 [29.9%] strains were cagA-positive, but cagE and cagT-negative). The simple gene as well as the combination of the genes in the cag PAI appeared not to be useful markers to predict H. pylori-related diseases in Iranian population. The genomic sequences of the cag PAI in Iranian strains might be considerably different from those in other geographic locations.
登录
查看更多内容
影响因子：
29.4
作者：
Naito, M;Yamazaki, T;Hatakeyama, M
通讯作者：
Hatakeyama, M
影响因子：
3.4
作者：
Backert, S;Ziska, E;Meyer, TF
通讯作者：
Meyer, TF
影响因子：
24.5
作者：
Maeda, S;Ogura, K;Omata, M
通讯作者：
Omata, M
影响因子：
24.5
作者：
Parsonnet, J;Friedman, GD;Vogelman, H
通讯作者：
Vogelman, H
DOI：
10.1073/pnas.93.25.14648
发表时间：
1996-12-10
影响因子：
11.1
作者：
Censini, S;Lange, C;Covacci, A
通讯作者：
Covacci, A