Cytokine gene transcription in vascularised organ grafts: analysis using semiquantitative polymerase chain reaction.
Cytokine gene transcription in vascularised organ grafts: analysis using semiquantitative polymerase chain reaction.
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DOI：
10.1084/jem.174.2.493
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发表时间：
1991-08-01
期刊：
影响因子：
--
通讯作者：
Morris PJ
中科院分区：
文献类型：
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作者：
Dallman MJ;Larsen CP;Morris PJ
通过直接分析小鼠异位心脏移植获得的RNA，分析了细胞因子基因的转录。半定量聚合酶链式反应(PCR)检测细胞因子基因的表达水平。被调查的细胞因子的表达分为三组。第一组包括IL-1β(IL-1β)、IL-5、IL-6和干扰素-γ(干扰素-γ)。这些基因在正常心脏组织中低水平表达，在同基因和同种异体移植后表达上调。第二组基因(IL-1α、IL-3)在正常心脏中未检测到表达水平，但在同基因或异基因心脏移植后诱导表达。第三组包括IL-2、IL-4和肿瘤坏死因子β，这些细胞因子仅在移植后的同种异体移植物中表达。
Cytokine gene transcription has been analyzed by direct analysis of RNA obtained from mouse heterotopic cardiac transplants. The level of expression of the cytokine genes was assessed using semiquantitative polymerase chain reaction (PCR). Expression of the cytokines investigated fell into three groups. The first group included interleukin 1 beta (IL-1 beta), IL-5, IL-6, and interferon gamma (IFN gamma). These genes were expressed in normal heart tissue at low level and were upregulated following both syngeneic and allogeneic transplantation. Genes in the second group (IL-1 alpha, IL-3) were not expressed at detectable levels in normal heart but were induced following either syngeneic or allogeneic heart grafting. IL-2, IL-4, and tumor necrosis factor beta (IFN beta) comprised the third group and these cytokines were expressed only in allogeneic grafts after transplantation.