PROPERTIES AND GENETIC-CONTROL OF 4 METHYLTRANSFERASES INVOLVED IN METHYLATION OF ANTHOCYANINS IN FLOWERS OF PETUNIA-HYBRIDA
PROPERTIES AND GENETIC-CONTROL OF 4 METHYLTRANSFERASES INVOLVED IN METHYLATION OF ANTHOCYANINS IN FLOWERS OF PETUNIA-HYBRIDA
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DOI：
10.1007/bf00392867
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发表时间：
1984-01-01
期刊：
影响因子：
4.3
通讯作者：
SCHRAM, AW
中科院分区：
文献类型：
--
作者：
JONSSON, LMV;AARSMAN, MEG;SCHRAM, AW
采用色谱聚焦技术分离了花中4个s -腺苷- l-蛋氨酸：花青素-3',5' - o -甲基转移酶。每个甲基转移酶由甲基化基因Mt1、Mt2、Mf1或Mf2中的一个控制。测定了4种酶在Mg2+、EDTA和s -腺苷- l-同型半胱氨酸存在下的分子量、ph -活性最佳值、等电点、若干动力学性质和行为。delphinidin 3-（对香豆醇）- rutinosido5 -glucoside的体外甲基化反映了体内甲基化花青素的积累模式，并确立了甲基转移酶在体内的调节作用。
Four S-adenosyl-L-methionine:anthocyanin-3'',5''-O-methyltransferases in flowers of P. hybrida were separated using the chromatofocusing technique. Each methyltransferase is controlled by one of the methylation genes Mt1, Mt2, Mf1 or Mf2. Molecular weight, pH-activity optimum, isoelectric point, several kinetic properties and the behavior in the presence of Mg2+, EDTA and S-adenosyl-L-homocysteine of each of the 4 enzymes were determined. The methylation in vitro of delphinidin 3-(p-coumaroyl)-rutinosido-5-glucoside reflected the accumulation patterns of methylated anthocyanins in vivo and established the regulatory role of methyltransferases in vivo.