Capsaicin partially mimics heat in mouse fibroblast cells in vitro.
Capsaicin partially mimics heat in mouse fibroblast cells in vitro.
复制标题
辣椒素在体外部分模拟小鼠成纤维细胞的热量。
DOI：
10.1007/s00210-016-1331-6
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
Shido O.
中科院分区：
文献类型：
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作者：
Sugimoto N;Katakura M;Matsuzaki K;Nakamura H;Yachie A;Shido O.
辣椒素激活瞬时受体电位香草酸1（TRPV1），瞬时受体电位家族中的阳离子通道，导致Ca2+和Mg2+的瞬时进入和温暖的感觉。然而，辣椒素对细胞的影响尚未完全阐明成纤维细胞。在本研究中，我们研究了辣椒素是否能诱导小鼠成纤维细胞的信号转导，并与热诱导的信号转导的效果进行了比较。在NIH3T3小鼠成纤维细胞中，分析了丝裂原活化蛋白激酶（MAPK）ERK和p38 MAPK的活化、热休克蛋白70（HSP70）和HSP90的表达水平、肌动蛋白组装和细胞增殖。用辣椒素（100 μM）刺激15分钟，磷酸化ERK和p38 MAPK并诱导肌动蛋白组装。用辣椒素刺激2天可增加HSP70的水平，但不增加HSP90的水平，用辣椒素（100 μM）刺激2天不影响细胞增殖。暴露于中等温度（39.5 °C）15分钟使ERK和p38 MAPK磷酸化，并诱导肌动蛋白组装，其程度与辣椒素刺激相似。2天暴露于中等热量增加了HSP 70和HSP 90的水平，并阻止了细胞增殖。然而，用辣椒素（100 μM）刺激2天未能阻止热休克诱导的细胞死亡。因此，我们的研究结果表明，辣椒素对成纤维细胞的影响部分不同于热。值得注意的是，用辣椒素刺激2天不足以在成纤维细胞中形成耐热性。
Capsaicin activates transient receptor potential vanilloid 1 (TRPV1), a cation channel in the transient receptor potential family, resulting in the transient entry of Ca2+and Mg2+and a warm sensation. However, the effects of capsaicin on cells have not fully elucidated in fibroblasts. In this study, we investigated whether capsaicin could induce signal transduction in mouse fibroblast cells and compared the effect with that of heat-induced signal transduction. The activation of the mitogen-activated protein kinases (MAPKs) ERK and p38 MAPK, expression levels of heat shock protein 70 (HSP70) and HSP90, actin assembly, and cell proliferation were analyzed in NIH3T3 mouse fibroblast cells. A 15-min stimulation with capsaicin (∼100 μM) phosphorylated ERK and p38 MAPK and induced actin assembly. A 2-day stimulation with capsaicin increased the level of HSP70, but not HSP90, and the 2-day stimulation with capsaicin (∼100 μM) did not affect cell proliferation. A 15-min exposure to moderate heat (39.5 °C) phosphorylated both ERK and p38 MAPK and induced actin assembly to similar degrees as stimulation with capsaicin. A 2-day exposure to moderate heat increased the levels of both HSP70 and HSP90 and prevented cell proliferation. However, the 2-day stimulation with capsaicin (100 μM) failed to prevent heat shock-induced cell death. Thus, our results suggest that the effects of capsaicin on fibroblast cells partially differ from those of heat. Notably, the 2-day stimulation with capsaicin was not sufficient to develop heat tolerance in fibroblast cells.