Viral and cellular fos proteins: A comparative analysis
Viral and cellular fos proteins: A comparative analysis
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DOI：
10.1016/0092-8674(84)90219-8
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发表时间：
1984-02
期刊：
影响因子：
64.5
通讯作者：
A. Curran;Dusty A. Miller;L. Zokas;I. Verma
中科院分区：
文献类型：
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作者：
A. Curran;Dusty A. Miller;L. Zokas;I. Verma
FBJ鼠骨肉瘤病毒（FBJ-MuSV）在小鼠中诱导骨肉瘤并在体外转化成纤维细胞。它含有一种称为vfos的致癌基因，该基因通过与相关辅助病毒重组而衍生自正常细胞基因。v-fos基因的产物是55，000道尔顿的蛋白质，约55“"。这种蛋白质存在于含有扩增水平的v-fos基因的细胞核中，也存在于病毒转化细胞的细胞核中。c-fos蛋白定位于正常小鼠羊膜细胞的细胞核和表达c-fos基因产物的重组质粒转化的细胞的细胞核中。然而，~ 55 'OS在核中比p55'经历更广泛的翻译后修饰。““.免疫荧光数据表明正常小鼠羊膜细胞中~ 55 μ g/ml的水平与在由v-fos或c-fos蛋白转化的成纤维细胞中发现的水平相似。
The FBJ murine osteosarcoma virus (FBJ-MuSV) induces osteosarcomas in mice and transforms fibroblasts in vitro. It contains an oncogene termed vfos derived from a normal cellular gene by recombination with an associated helper virus. The product of the v-fos gene is a 55,000 dalton protein,~ 55”‘““. This protein was found in the nuclei of cells containing amplified levels of the v-fos gene, and also in the nuclei of virus-transformed cells. The c-fos protein was localized in the nuclei of normal mouse amnion cells and in the nuclei of cells transformed by a recombinant plasmid that expresses the c-fos gene product. However,~ 55”‘OS undergoes more extensive post-translational modification in the nucleus than p55”.‘““. lmmunofluorescence data indicate that the level of~ 55”‘“” in normal mouse amnion cells is similar to that found in fibroblasts transformed by the v-fos or c-fos proteins.