Design and performance of a low-flow capillary electrophoresis-electrospray-mass spectrometry interface using an emitter with dual beveled edge
Design and performance of a low-flow capillary electrophoresis-electrospray-mass spectrometry interface using an emitter with dual beveled edge
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DOI：
10.1002/elps.200410159
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发表时间：
2005-04-01
期刊：
影响因子：
2.9
通讯作者：
Her, GR
中科院分区：
文献类型：
--
作者：
Chen, YR;Tseng, MC;Her, GR
建立了毛细管区带电泳（CZE）与电喷雾电离质谱（ESI-MS）相结合的低流量电泳质谱（CE-MS）界面。界面由两个平行的毛细管柱（分离柱和上料柱）和一个双斜边发射器组成。在保持相对较低的最佳流量的同时，双斜边ESI发射器允许使用具有较大孔的尖端。因此，与扁平尖端相比，该接口不太容易出现列阻塞。由于样品稀释度低，初始液滴更小，该界面比传统的鞘液界面具有更好的灵敏度。此外，发现该界面对非挥发性盐的存在具有更强的抵抗力。以40 mm硼酸盐和20 mm α -环糊精（α - cd）为缓冲液，用CE-MS检测了4种主要形式的神经节苷脂。
A low-flow electrophoresis-mass spectrometry (CE-MS) interface has been developed for interfacing capillary zone electrophoresis (CZE) with electrospray- ionization-mass spectrometry (ESI-MS). The interface consists of two parallel capillary columns (a separation column and a makeup column), and an emitter with a dual beveled edge. While maintaining a relatively low optimum flow rate, the dual-beveled-edge ESI emitter allows the use of a tip with larger orifice. Therefore, this interface is less prone to column blocking in comparison with a flat tip. Primarily attributed to low sample dilution and smaller initial droplet, the interface showed better sensitivity than a conventional sheath liquid interface. Furthermore, the interface was found to be more resistant to the presence of nonvolatile salts. By using 40 mm borate and 20 mm alpha-cyclodextrin (alpha-CD) as the running buffer, four major forms of gangliosides were detected by CE-MS.