Expression and immunolocalization of p59c-fyn tyrosine kinase in rat eggs.
Expression and immunolocalization of p59c-fyn tyrosine kinase in rat eggs.
复制标题
大鼠卵中 p59c-fyn 酪氨酸激酶的表达和免疫定位。
DOI：
10.1006/dbio.1997.8816
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发表时间：
1998
影响因子：
2.7
通讯作者：
Shalgi,R
中科院分区：
文献类型：
--
作者：
Talmor,A;Kinsey,WH;Shalgi,R
受精通过触发一系列生化事件克服减数分裂停滞，导致卵的激活。在无脊椎动物和低等脊椎动物的卵中已经鉴定出一小组蛋白酪氨酸激酶（PTKs），抑制剂研究表明它们在卵激活的晚期事件中起作用。最近一项使用海胆系统的研究表明，Fyn激酶在卵子中表达，并在受精后几分钟内被激活。在本研究中，蛋白质印迹分析以及免疫复合物激酶试验表明，p59 c-fynkinase表达在未受精和受精的大鼠卵子。免疫荧光共聚焦显微镜表明，Fyn激酶定位于卵皮质，但也从激活的卵的皮质细胞质中升高的极体和视锥。令人惊讶的是，Fyn也被发现高度集中在减数分裂和有丝分裂纺锤体上。迄今为止，Fyn是第一个被证明存在于哺乳动物卵子中的PTK。Fyn定位于卵皮质以及纺锤体微管表明这种蛋白激酶在卵内可能具有多种功能。
Fertilization overcomes meiotic arrest by triggering a series of biochemical events, resulting in activation of the egg. A small group of protein tyrosine kinases (PTKs) have been identified in eggs of invertebrates and lower vertebrates and inhibitor studies have suggested that they play a role in late events of egg activation. A recent study using the sea urchin system demonstrated that Fyn kinase was expressed in eggs and was activated within minutes of fertilization. In the present study, Western blot analysis as well as immune complex kinase assay demonstrated that p59c-fynkinase was expressed in both unfertilized and fertilized rat eggs. Immunofluorescence confocal microscopy demonstrated that Fyn kinase was localized to the egg cortex but also to the polar body and the fertilizing cone which are elevated from the cortical cytoplasm of the activated egg. Surprisingly, Fyn was also found to be highly concentrated over the meiotic and mitotic spindles. To date, Fyn is the first PTK demonstrated to be present in the mammalian egg. Localization of Fyn to the egg cortex as well as the spindle microtubules indicates that this protein kinase may have multiple functions within the egg.