FLUOROMETRIC ASSAY OF HUMAN-SERUM CARNOSINASE ACTIVITY IN NORMAL-CHILDREN, ADULTS AND PATIENTS WITH MYOPATHY
FLUOROMETRIC ASSAY OF HUMAN-SERUM CARNOSINASE ACTIVITY IN NORMAL-CHILDREN, ADULTS AND PATIENTS WITH MYOPATHY
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DOI：
10.1177/000456328402100613
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发表时间：
1984-01-01
影响因子：
2.2
通讯作者：
MIYAI, K
中科院分区：
文献类型：
--
作者：
BANDO, K;SHIMOTSUJI, T;MIYAI, K
建立了一种灵敏、准确的测定血清肌肽酶的方法。用此方法测定了正常成人、婴儿和儿童的血清值。小于1岁的婴儿的平均值非常低，但随着年龄的增长而增加，6岁或6岁以上的儿童与成人几乎相同。在成年男性中，平均活性为1.85 μ mol/ml/h，在成年女性中为2.07 μ mol/ml/h。在肌营养不良症患者中观察到低活动。
A sensitive, accurate method has been established for the assay of serum carnosinase by measuring the fluorescence emitted from the L-histidine liberated on treatment with o-phthaldialdehyde. Using this method the serum values for normal adults, infants and children were measured. The mean value was very low in infants of less than 1 yr old but increased with age, being almost the same in children aged 6 yr or more as in adults. In adult men, the mean activity was 1.85 .mu.mol/ml/h and in adult women it was 2.07 .mu.mol/ml/h. Low activity was observed in patients with muscular dystrophy.