Kruppel homolog 1, an early juvenile hormone-response gene downstream of Methoprene-tolerant, mediates its anti-metamorphic action in the red flour beetle Tribolium castaneum
Kruppel homolog 1, an early juvenile hormone-response gene downstream of Methoprene-tolerant, mediates its anti-metamorphic action in the red flour beetle Tribolium castaneum
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DOI：
10.1016/j.ydbio.2008.10.016
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发表时间：
2009-01-15
影响因子：
2.7
通讯作者：
Shinoda, Tetsuro
中科院分区：
文献类型：
--
作者：
Minakuchi, Chieka;Namiki, Toshiki;Shinoda, Tetsuro
保幼激素（JH）可防止蜕皮激素诱导的昆虫变态。然而，我们的知识的分子机制JH行动仍然是支离破碎的。Kruppel同源物1（Kr-h1）是黑腹果蝇（Drosophila melanogaster）中的JH诱导型转录因子（Minakuchi，C.，Zhou，X.，中国科学院，Riddiford，L.M.，2008年b。Kruppel同源物1（Kr-h1）在果蝇变态过程中介导保幼激素的作用。机械设备125，91-105）。对赤拟谷盗同源基因TcKr-h1的表达分析表明，其转录本在赤拟谷盗幼虫期持续存在，但在蛹期不存在。RNAi介导的TcKr-h1在幼虫阶段的敲低诱导早熟的幼虫蛹转变。在蛹的早期阶段，用外源JH模拟物（OHM）处理引起第二蛹的形成，以及TcKr-h1转录的快速和大量诱导。JHM诱导的第二蛹的形成被TcKr-h1的敲低所抵消。结合JHM处理的RNAi实验表明，在幼虫阶段，TcKr-h1在推定的JH受体Methoprene-tolerant（TcMet）的下游起作用，并且在蛹阶段，它在TcMet的下游和蛹特异性宽（Tcbr）的上游起作用。因此，TcKr-h1是介导JH作用的早期JH反应基因，其连接TcMet和Tcbr。(C)2008年爱思唯尔公司All rights reserved.
Juvenile hormone (JH) prevents ecdysone-induced metamorphosis in insects. However, our knowledge of the molecular mechanisms of JH action is still fragmented. Kruppel homolog 1 (Kr-h1) is a JH-inducible transcription factor in Drosophila melanogaster (Minakuchi, C., Zhou, X., Riddiford, L.M., 2008b. Kruppel homolog 1 (Kr-h1) mediates juvenile hormone action during metamorphosis of Drosophila melanogaster. Mech. Dev. 125, 91-105). Analysis of expression of the homologous gene (TcKr-h1) in the beetle Tribolium castaneum showed that its transcript was continuously present in the larval stage but absent in the pupal stage, Artificial suppression of JH biosynthesis in the larval stage caused a precocious larval-pupal transition and a down-regulation of TcKr-h1 mRNA. RNAi-mediated knockdown of TcKr-h1 in the larval stage induced a precocious larval-pupal transition. In the early pupal stage, treatment with an exogenous JH mimic OHM) caused formation of a second pupa, and a rapid and large induction of TcKr-h1 transcription. JHM-induced formation of a second pupa was counteracted by the knockdown of TcKr-h1. RNAi experiments in combination with JHM treatment demonstrated that in the larval stage TcKr-h1 works downstream of the putative JH receptor Methoprene-tolerant (TcMet), and in the pupal stage it works downstream of TcMet and upstream of the pupal specifier broad (Tcbr). Therefore, TcKr-h1 is an early JH-response gene that mediates JH action linking TcMet and Tcbr. (C) 2008 Elsevier Inc. All rights reserved.