Lipolytic activity of Campylobacter pylori: effect of colloidal bismuth subcitrate (De-Nol)
Lipolytic activity of Campylobacter pylori: effect of colloidal bismuth subcitrate (De-Nol)
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发表时间：
1989-10
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通讯作者：
B. Slomiany;Chinnaswamy Kasinathan;A. Slomiany
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作者：
B. Slomiany;Chinnaswamy Kasinathan;A. Slomiany
幽门螺杆菌感染似乎在胃病的病因学中起主要作用，但这种病原体在胃粘膜防御中引起损害的性质尚不清楚。我们在这里提出的证据表明，这种微生物制备的细胞外物质表现出能够降解胃粘膜脂质的溶脂活性。从内镜患者的胃窦粘膜活检标本中培养菌落，用生理盐水洗涤，通过灭菌过滤器，并检测滤液的脂肪酶和磷脂酶活性。通过跟踪三油酸甘油和双棕榈酰磷脂酰胆碱的降解，我们确定了脂肪酶和磷脂酶A的存在。甘油三酯降解的主要产物是单油酸甘油，而溶血磷脂酰胆碱是由磷脂酰胆碱降解产生的。抗溃疡药物De-Nol可抑制幽门螺杆菌滤液的溶脂活性，其在150微克/毫升时可使脂肪酶活性降低21%，磷脂酶a活性降低60%。结果表明，De-Nol能够阻止幽门螺杆菌脂肪酶对粘膜脂质的降解，从而有助于保持粘膜的完整性。
Infection by Campylobacter pylori appears to play a major role in the etiology of gastric disease, but the nature of impairment evoked by this pathogen in gastric mucosal defense is not well understood. We present here evidence that the extracellular material elaborated by this microorganism exhibits lipolytic activity capable of gastric mucosal lipid degradation. The colonies of bacteria, cultured from antral mucosal biopsy specimens of patients undergoing endoscopy, were washed with saline, passed through a sterilization filter, and the filtrate was examined for lipase and phospholipase activities. By following the degradation of glycerol trioleate and dipalmitoyl phosphatidylcholine, we established the presence of lipase and phospholipase A enzymes. The major product of the triglyceride degradation was glycerol monooleate, while lysophosphatidylcholine resulted from the degradation of phosphatidylcholine. The lipolytic activity of C. pylori filtrate was inhibited by an antiulcer drug, De-Nol, which at 150 micrograms/ml caused a 21% reduction in lipase activity and a 60% reduction in the activity of phospholipase A. The results suggest that De-Nol is capable of preventing degradation of mucosal lipids by C. pylori lipases and, hence, helps to preserve the mucosal integrity.