Influence of the surface structure of titanium materials on the adhesion of fibroblasts
Influence of the surface structure of titanium materials on the adhesion of fibroblasts
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DOI：
10.1016/0142-9612(96)87281-4
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发表时间：
1996-07-01
期刊：
影响因子：
14
通讯作者：
Breme, J
中科院分区：
文献类型：
--
作者：
Eisenbarth, E;Meyle, J;Breme, J
对于植入物的成功使用来说，最重要的是周围组织与生物材料的良好粘附，除了植入物的表面组成外，表面形貌也影响粘附细胞的特性。本研究的目的是利用荧光显微镜研究基质的表面结构对培养的牙龈成纤维细胞的形成和取向的影响。进一步的目标是确定材料成分对细胞形状的影响，假设在每种情况下，细胞的延长可以预期提供比球形细胞形状更有利的粘附行为。为了描述细胞的形状，定义了一个形状因子，该因子由细胞所覆盖的面积和它们的周长计算得到。为了确定表面结构的影响，对cp-titanium、TiAl6V4和TiTa30的基体薄片进行研磨。在这些标本上培养第5 ~ 7代人牙龈成纤维细胞。培养两天后，细胞固定并染色。定向细胞的数量被确定为衬底表面粗糙度的函数。定向细胞的数量随着基底粗糙度的增加而增加（与材料无关）。在抛光表面上，定向细胞的数量为11%（峰谷平均高度为0.04 μ m）；峰谷高度为1.36 μ m时，定向细胞的数量增加到72%。可以观察到，定向细胞在与磨槽边缘接触处具有更高的聚焦接触密度。为了确定表面组成对牙龈成纤维细胞的影响，我们将成纤维细胞在cp-钛、TiAl6V4和TiTa30的抛光基质标本上培养14 d，观察其形状的差异。在cp-titanium和TiTa30上生长的细胞形状因子分别为1.76和1.58，而在TiAl6V4上生长的细胞形状因子为0.93。培养14d后，在TiAl6V4上生长的成纤维细胞呈不利的球形（导致附着力差），这可能是底物中局部钒浓度增加的结果，同时有毒钒离子的释放也随之增加。(C) 1996 Elsevier Science Limited
Of utmost importance for the successful use of an implant is a good adhesion of the surrounding tissue to the biomaterial, in addition to the surface composition of the implant, the surface topography also influences the properties of the adherent cells. The aim of this investigation was thus to study the influence of the surface structure of the substrate on the formation of focal contacts and on the orientation of cultivated gingival fibroblasts by means of fluorescence microscopy. A further goal was to determine the effect of the material composition on the cell shape, on the assumption that in each case a lengthening of the cells can be expected to provide a more favourable adhesion behaviour than a spherical cell shape. In order to describe the shape of the cell, a shape factor was defined which was calculated from the area cove red by the cells and from their circumference. To determine the influence of the surface structure, substrate platelets of cp-titanium, TiAl6V4 and TiTa30 were ground. Onto these specimens hu man gingival fibroblasts of the 5th to 7th passages were cultivated. After a culture time of two days the cells were fixed and stained. The number of orientated cells was determined as a function of the surface roughness of the substrate. The number of orientated cells was shown to increase - independent of the material - with increasing roughness of the ground substrate. On a polished surface the number of orientated cells was 11% (average peak-to-valley height 0.04 mu m); at a peak-to-valley height of 1.36 mu m the number of orientated cells increased to 72%. It could be observed that the orientated cells had a higher density of focal contacts where they were in contact with the edges of the grinding grooves. In order to determine the effect of the surface composition, gingival fibroblasts were cultured for 14 d on polished substrate specimens of cp-titanium, TiAl6V4 and TiTa30 and examined for differences in shape. The cells grown on cp-titanium and on TiTa30 had shape factors of 1.76 and 1.58 respectively, whereas those grown on TiAl6V4 had a shape factor of 0.93. The unfavourable spherical shape of the fibroblasts (resulting in a poor adhesion) grown on TiAl6V4 after a culture period of 14 d may be the result of a locally increased vanadium concentration in the substrate, with an accompanying increase in the release of toxic vanadium ions. (C) 1996 Elsevier Science Limited