Lack of association between polymorphisms in the P2X7 gene and tuberculosis in a Chinese Han population
Lack of association between polymorphisms in the P2X7 gene and tuberculosis in a Chinese Han population
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DOI：
10.1111/j.1574-695x.2008.00508.x
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发表时间：
2009-01-01
影响因子：
--
通讯作者：
Shen, Adong
中科院分区：
文献类型：
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作者：
Xiao, Jing;Sun, Lin;Shen, Adong
一些研究表明，遗传因素可能会影响人群对结核病的易感性，并且已经发现P2 X7与一些西非，东南亚，北美和北欧人群中结核病的风险增加有关。为了探讨P2 X7在中国汉族人群结核病易感性中的潜在作用，我们评估了P2 X7的1513 A-> C和-762T -> C多态性与结核病风险的关系。PCR扩增基因组DNA，然后进行限制性片段长度多态性分析，并使用等位基因特异性PCR。我们发现96例结核病患者与384例对照组相比，1513 A-> C多态性的基因型和等位基因频率无显著差异[P=0.856和0.316; C等位基因的比值比（OR）=0.976; 95%置信区间（CI）=0.755-1.262]。同样，-762 T-> C多态性与结核病之间也没有发现显著相关性（患者和对照组的P值分别为0.102和0.095; C等位基因的OR =0.924; 95%CI =0.847-1.010）。因此，我们对P2 X7的分析表明，1513 A-> C和-762 T-> C多态性似乎与中国汉族人群对结核病的易感性无关。
Several studies have suggested that genetic factors may affect the susceptibility of a population to tuberculosis, and it has been found that P2X7 is linked to an increased risk for tuberculosis in some West African, Southeast Asian, North American, and North European populations. To explore the potential role of P2X7 in the susceptibility to tuberculosis among members of the Chinese Han population, we evaluated the association of the 1513A -> C and -762T -> C polymorphisms in P2X7 with the risk for tuberculosis. PCR amplification of genomic DNA was followed by restriction fragment length polymorphism analysis, and allele-specific PCR was used. We found no significant differences in the genotypic and allelic frequencies of 1513A -> C polymorphisms in 96 patients with tuberculosis compared with 384 control subjects [P=0.856 and 0.316, respectively; odds ratio (OR) for the C allele=0.976; 95% confidence interval (CI)=0.755-1.262]. Similarly, no significant association was found between the -762T -> C polymorphism and tuberculosis (P=0.102 and 0.095 for the patients and controls, respectively; OR for the C allele=0.924; 95% CI=0.847-1.010). Thus, our analysis of P2X7 showed that the 1513A -> C and -762T -> C polymorphisms did not appear to be associated with the susceptibility of the Chinese Han population to tuberculosis.