Characterization of Small Isotropic BiceIles with Various Compositions
Characterization of Small Isotropic BiceIles with Various Compositions
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DOI：
10.1021/acs.langmuir.6b00867
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发表时间：
2016-07-05
期刊：
影响因子：
3.9
通讯作者：
Arseniev, A. S.
中科院分区：
文献类型：
--
作者：
Mineev, K. S.;Nadezhdin, K. D.;Arseniev, A. S.
膜蛋白的结构研究是非常重要和感兴趣的，溶液和固态NMR光谱是非常有前途的工具，这项任务。然而，这种研究受到许多障碍的阻碍，首先是膜蛋白需要模拟细胞膜的适当环境。各向同性双胞膜是目前应用最广泛、最有前景的膜模拟物之一。虽然大的各向异性双胞被很好地研究，但小双胞的场包含许多“白色斑点”。本工作报告了由1，2-二己酰-sn-甘油-3-磷酸胆碱（DHPC）、3-[（3-胆酰胺丙基）二甲基铵基]-1-丙磺酸盐（CHAPS），3- [（3-胆酰胺丙基）二甲基铵基]-2-羟基-1-丙磺酸盐（CHAPS 0）和胆酸钠，作为脂质/去污剂比率和温度的函数。这些参数的测量使用1H NMR扩散光谱的双胞组成的脂质与饱和脂肪链的不同长度和脂质，含有不饱和脂肪酸残基。用异核NMR和核Overhauser效应谱研究了模型跨膜蛋白（大鼠TrkA的膜结构域）对双胞性质的影响以及双胞大小和组成对跨膜蛋白性质的影响。我们表明，适用于溶液NMR光谱的各向同性bicelles的行为所预测的理论模型，很可能是bicelles，而不是混合胶束。使用所获得的数据，我们提出了一个简单的方法来控制在低浓度下的大小bicelles。在我们的研究结果的基础上，我们比较了不同的边缘形成剂，并选择CHAPS作为洗涤剂的选择，在bicelles的结构研究，如果不需要的氘化的洗涤剂。
Structural studies of membrane proteins are of great importance and interest, with solution and solid state NMR spectroscopy being very promising tools for that task. However, such investigations are hindered by a number of obstacles, and in the first place by the fact that membrane proteins need an adequate environment that models the cell membrane. One of the most widely used and prospective membrane mimetics is isotropic bicelles. While large anisotropic bicelles are well studied, the field of small bicelles contains a lot of "white spots". The present work reports the radii of particles and concentration of the detergents in the monomeric state in solutions of isotropic bicelles, formed by 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC), 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS), 3- [ (3-cholamidopropyl) dimethylammonio]-2-hydroxy-1-propanesulfonate (CHAPS 0), and sodium cholate, as a function of lipid/detergent ratio and temperature. These parameters were measured using ill NMR diffusion spectroscopy for the bicelles composed of lipids with saturated fatty chains of different length and lipids, containing unsaturated fatty acid residue. The influence of a model transmembrane protein (membrane domain of rat TrkA) on the properties of bicelles and the effect of the bicelle size and composition on the properties of the transmembrane protein were investigated with heteronudear NMR and nuclear Overhauser effect spectroscopy. We show that isotropic bicelles that are applicable for solution NMR spectroscopy behave as predicted by the theoretical models and are likely to be bicelles rather than mixed micelles. Using the obtained data, we propose a simple approach to control the size of bicelles at low concentrations. On the basis of our results, we compared different rim-forming agents and selected CHAPS as a detergent of choice for structural studies in bicelles, if the deuteration of the detergent is not required.