Elevated expression of PDI family proteins during differentiation of mouse F9 teratocarcinoma cells
Elevated expression of PDI family proteins during differentiation of mouse F9 teratocarcinoma cells
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小鼠 F9 畸胎癌细胞分化过程中 PDI 家族蛋白表达升高
DOI：
--
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发表时间：
1998
影响因子：
4
通讯作者：
S. Saga
中科院分区：
文献类型：
--
作者：
O. Miyaishi;K. Kozaki;K. Iida;Kensuke Isobe;Y. Hashizume;S. Saga
我们研究了蛋白质二硫键异构酶家族蛋白质（PDI，ERp 61和ERp 72）在小鼠F9畸胎瘤细胞分化过程中诱导维甲酸和二丁酰cAMP处理的表达。该家族的每个成员在未分化的F9细胞中以组成型水平表达。在分化的F9细胞壁或内脏内胚层细胞的所有这些酶的蛋白质水平增加，虽然这种增加的程度在蛋白质和mRNA水平之间的酶变化。发现某些蛋白质在化学交联剂存在下与PDI、ERp 61和ERp 72共免疫沉淀。IV型胶原蛋白与PDI显着共沉淀，而层粘连蛋白同样与三种蛋白质共沉淀。此外，210 kDa的蛋白质特征性地与ERp 72共沉淀。因此，在F9细胞分化过程中PDI家族蛋白的诱导及其与不同蛋白的结合可能暗示该家族每个成员的特定功能，尽管共同的氧化还原活性能够催化二硫键形成。J.细胞。68：436-445，1998.© 1998 Wiley利斯公司
We investigated the expression of protein disulfide isomerase family proteins (PDI, ERp61, and ERp72) in mouse F9 teratocarcinoma cells during differentiation induced by treatment with retinoic acid and dibutyryl cAMP. Each member of this family was expressed at a constitutive level in undifferentiated F9 cells. During differentiation of F9 cells to parietal or visceral endodermal cells the protein level of all these enzymes increased, although the extent of this increase in both protein and mRNA levels varied among the enzymes. Certain proteins were found to be co‐immunoprecipitated with PDI, ERp61, and ERp72 in the presence of a chemical crosslinker. Type IV collagen was significantly coprecipitated with PDI whereas laminin was equally coprecipitated with the three proteins. Furthermore, 210 kDa protein characteristically coprecipitated with ERp72. Thus, the induction of PDI family proteins during the differentiation of F9 cells and their association with different proteins may implicate specific functions of each member of this family despite the common redox activity capable of catalyzing the disulfide bond formation. J. Cell. Biochem. 68:436–445, 1998. © 1998 Wiley‐Liss, Inc.
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DOI：
10.1016/s0021-9258(17)37352-0
发表时间：
1994-03
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Alberto Puig;H. Gilbert
通讯作者：
Alberto Puig;H. Gilbert
DOI：
10.1016/s0021-9258(18)88868-8
发表时间：
1985-06
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
M. Lewis;S. Turco;M. Green
通讯作者：
M. Lewis;S. Turco;M. Green
大鼠肝微粒体中硫醇新同工酶：蛋白质二硫键氧化还原酶的纯化和表征。
DOI：
--
发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Srivastava,SP;Chen,NQ;Liu,YX;Holtzman,JL
通讯作者：
Holtzman,JL
影响因子：
2.7
作者：
S. Wang;G. Larosa;L. Gudas
通讯作者：
S. Wang;G. Larosa;L. Gudas