Identification of single nucleotide polymorphisms in the bovine CCL2, IL8, CCR2 and IL8RA genes and their association with health and production in Canadian Holsteins
Identification of single nucleotide polymorphisms in the bovine CCL2, IL8, CCR2 and IL8RA genes and their association with health and production in Canadian Holsteins
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DOI：
10.1111/j.1365-2052.2007.01588.x
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发表时间：
2007-06-01
期刊：
影响因子：
2.4
通讯作者：
Karrow, N. A.
中科院分区：
文献类型：
--
作者：
Leyva-Baca, I.;Schenkel, F.;Karrow, N. A.
本研究的目的是确定趋化因子基因CCL 2和IL 8以及趋化因子受体基因IL 8 RA和CCR 2中存在的SNPs，并评估其对加拿大荷斯坦公牛体细胞评分（SCS）和其他四个性状的估计育种值（EBV）变化的潜在贡献。使用SCS的具有高（H）和低（L）EBV的公牛的DNA测序来鉴定11个SNP。在CCL 2基因中发现了两个未报道的SNP，在CCR 2基因中发现了一个SNP。还鉴定了先前报道的SNP（三个在IL 8基因中，五个在IL 8 RA趋化因子受体中）。采用四引物ARMS-PCR对338头加拿大荷斯坦公牛CCL 2、IL 8、IL 8 RA和CCR 2的4个SNP位点进行了基因分型。我们调查了这七个多态性与三个生产性状（产奶量，脂肪产量和蛋白质产量）和一个构象性状相关的乳腺炎（乳房深度）。CCL 2 rs 41255713：T > C SNP的等位基因取代效应在实验水平上对于产奶量（247.5 +/-79.9 kg）和蛋白质产量（7.4 +/-2.3 kg）EBV是显著的（P <0.05）。SCS上的T SNP在比较水平上是显著的（-0.04 +/-0.02，P = 0.05），这可能表明支持其他已发表研究的可能关联。最后，我们将CCR 2分配给BTA 22 q24，其中先前鉴定了SCS的QTL。
The aim of this study was to identify the presence of SNPs in the chemokine genes CCL2 and IL8 and the chemokine receptor genes IL8RA and CCR2, and assess their potential contribution to variation in estimated breeding values (EBVs) for somatic cell score (SCS) and four other traits in Canadian Holstein bulls. Pools of DNA for bulls with high (H) and low (L) EBVs for SCS were used for identification of 11 SNPs. Two unreported SNPs were found in the CCL2 gene and one SNP was found in the CCR2 gene. Previously reported SNPs (three in the IL8 gene and five in the IL8RA chemokine receptor) were also identified. Two SNPs in CCL2, three in IL8, one in IL8RA and one in CCR2 were genotyped in Canadian Holstein bulls (n = 338) using tetra primer ARMS-PCR. We investigated associations of these seven polymorphisms with three production traits (milk yield, fat yield and protein yield) and one conformation trait related to mastitis (udder depth). The allele substitution effect for the CCL2 rs41255713:T > C SNP was significant at an experimental-wise level for milk yield (247.5 +/- 79.9 kg) and protein yield (7.4 +/- 2.3 kg) EBVs (P T SNP on SCS was significant at the comparison-wise level (-0.04 +/- 0.02, P = 0.05), which might indicate a possible association in support of other published studies. Lastly, we assigned CCR2 to BTA22q24, where a previously QTL for SCS was identified.