Detection of Iron Depletion- and Hypoxia-Induced Mitophagy in Mammalian Cells
Detection of Iron Depletion- and Hypoxia-Induced Mitophagy in Mammalian Cells
复制标题
哺乳动物细胞中缺铁和缺氧诱导的线粒体自噬的检测
DOI：
10.1007/978-1-4939-7831-1_18
复制
发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Kanki Tomotake
中科院分区：
文献类型：
--
作者：
Yamashita Shun-ichi;Kanki Tomotake
线粒体自噬（Mitochondrial autophagy或mitophagy）是一种通过自噬选择性降解线粒体的过程。据信线粒体自噬降解受损或不必要的线粒体，并且对于维持线粒体稳态是重要的。迄今为止，已知几种刺激可以诱导线粒体自噬。然而，这些刺激（包括铁耗竭，缺氧和氮饥饿）诱导轻度线粒体自噬，这是很难通过测量线粒体质量的减少来检测。最近，我们已经成功地检测到在这些条件下使用mito-Keima作为报告诱导的线粒体自噬。在这一章中，我们描述了使用mito-Keima表达细胞诱导和检测铁耗竭和缺氧诱导的线粒体自噬的方案。
Mitochondrial autophagy or mitophagy is a process that selectively degrades mitochondria via autophagy. It is believed that mitophagy degrades damaged or unnecessary mitochondria and is important for maintaining mitochondrial homeostasis. To date, it is known that several stimuli can induce mitophagy. However, some of these stimuli (including iron depletion, hypoxia, and nitrogen starvation) induce mild mitophagy, which is difficult to detect by measuring the decrease in mitochondrial mass. Recently, we have successfully detected mitophagy induced under these conditions using mito-Keima as a reporter. In this chapter, we describe the protocols for induction and detection of iron depletion- and hypoxia-induced mitophagy using the mito-Keima-expressing cells.