IMPORTANCE OF 1,25-DIHYDROXYVITAMIN D-3 AND THE NONADHERENT CELLS OF MARROW FOR OSTEOBLAST DIFFERENTIATION FROM RAT MARROW STROMAL CELLS
IMPORTANCE OF 1,25-DIHYDROXYVITAMIN D-3 AND THE NONADHERENT CELLS OF MARROW FOR OSTEOBLAST DIFFERENTIATION FROM RAT MARROW STROMAL CELLS
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DOI：
10.1016/8756-3282(95)00099-y
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发表时间：
1995-06-01
期刊：
影响因子：
4.1
通讯作者：
LEBOY, PS
中科院分区：
文献类型：
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作者：
RICKARD, DJ;KAZHDAN, I;LEBOY, PS
虽然类固醇激素调节成熟成骨细胞的功能，但对其对成骨前体细胞的作用知之甚少，通过检测成骨细胞标志物碱性磷酸酶(AP)在大鼠骨髓基质细胞培养中的生长和诱导，探讨了类固醇激素在成骨细胞分化早期阶段调节的可能性。1，25(OH)(2)D-3可促进基质细胞的生长，而地塞米松、17β-雌二醇或维甲酸不能促进基质细胞的增殖和AP活性，1，25(OH)(2)D-3的促增殖作用是非贴壁细胞依赖的，BMP-2也可增加AP的水平，并与1，25(OH)(2)D-3协同作用。这些结果提示：(1)非贴壁的骨髓细胞可能支持基质细胞的发育，(2)1，25(OH)(2)D-3和糖皮质激素可能调节骨髓的成骨，但不支持雌激素的类似作用。
Although steroid hormones regulate mature osteoblast function, much less is known about their actions on osteoprogenitor cells, The possibility of steroid hormone regulation of early stages in osteoblast differentiation was investigated by measuring the growth and induction of the osteoblast marker enzyme alkaline phosphatase (AP) in rat bone marrow stromal cell cultures, Experiments were performed in charcoal-stripped serum; conditions which markedly impaired stromal cell growth, However, growth could be stimulated by nonadherent marrow cell-derived conditioned medium, 1,25(OH)(2)D-3, but not dexamethasone, 17 beta-estradiol, or retinoic acid, increased both stromal cell proliferation and AP activity, The increased proliferation with 1,25(OH)(2)D-3 was nonadherent cell-dependent, BMP-2 also increased AP levels and acted in synergy with 1,25(OH)(2)D-3. These results suggest that (i) nonadherent marrow cells may support stromal cell development, and (ii) 1,25(OH)(2)D-3 as well as glucocorticoids may regulate osteogenesis from the bone marrow but a similar role for estrogen is not supported.